FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1574672893> ?p ?o ?g. }

• W1574672893 endingPage "18752" @default.
• W1574672893 startingPage "18744" @default.
• W1574672893 abstract "Lactase-phlorizin hydrolase was isolated by immunoadsorption chromatography from rabbit brush-border membrane vesicles. Inactivation of the enzyme with [3H]conduritol-B-epoxide, a covalent active site-directed inhibitor, labeled glutamates at positions 1271 and 1747. Glu1271 was assigned to lactase, Glu1747 to phlorizin hydrolase activity. In contrast, the nucleophiles in the active sites of sucrase-isomaltase are aspartates (Asp505 and Asp1394). Asp505 is a part of the isomaltase active site and is localized on the larger subunit, which carries the membrane anchor also, while Asp1394 is a part of the active of sucrase. Alignment of these 2 nucleophilic residues in lactase-phlorizin hydrolase and of their flanking regions with published sequences of several other beta-glycosidases allows the classification of the configuration retaining glycosidases into two major families: the Asp and the Glu glycosidases, depending on the carboxylate presumed to interact with the putative oxocarbonium ion in the transition state. We offer some predictions as to the acting as the nucleophile in the active site of some glycosidases. By hydrophobic photolabeling, the membrane-spanning domain of lactase-phlorizin hydrolase was directly localized in the carboxyl-terminal region thus confirming this enzyme as a monotopic type I protein (i.e. with Nout-Cin orientation) of the brush-border membranes. A simplified version of the Me2+ precipitation method to efficiently and simply prepare brush-border membrane vesicles is also reported." @default.
• W1574672893 created "2016-06-24" @default.
• W1574672893 creator A5002666918 @default.
• W1574672893 creator A5043994505 @default.
• W1574672893 creator A5044505827 @default.
• W1574672893 creator A5082786845 @default.
• W1574672893 creator A5091724579 @default.
• W1574672893 date "1992-09-01" @default.
• W1574672893 modified "2023-09-30" @default.
• W1574672893 title "Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase. Comparison with sucrase-isomaltase and with other glycosidases, the membrane anchor of lactase-phlorizin hydrolase." @default.
• W1574672893 cites W1499586141 @default.
• W1574672893 cites W1503873442 @default.
• W1574672893 cites W1506033718 @default.
• W1574672893 cites W1522770871 @default.
• W1574672893 cites W1525347874 @default.
• W1574672893 cites W1552110160 @default.
• W1574672893 cites W1566240917 @default.
• W1574672893 cites W1568164430 @default.
• W1574672893 cites W1586096959 @default.
• W1574672893 cites W1588544209 @default.
• W1574672893 cites W1596721279 @default.
• W1574672893 cites W1605058617 @default.
• W1574672893 cites W1605271364 @default.
• W1574672893 cites W1625674217 @default.
• W1574672893 cites W1708604775 @default.
• W1574672893 cites W1764725331 @default.
• W1574672893 cites W1804060812 @default.
• W1574672893 cites W1963502824 @default.
• W1574672893 cites W1964917157 @default.
• W1574672893 cites W1965676881 @default.
• W1574672893 cites W1966551225 @default.
• W1574672893 cites W1968251312 @default.
• W1574672893 cites W1975085160 @default.
• W1574672893 cites W1981009721 @default.
• W1574672893 cites W1983035697 @default.
• W1574672893 cites W1985224958 @default.
• W1574672893 cites W1986412439 @default.
• W1574672893 cites W200081977 @default.
• W1574672893 cites W2002079142 @default.
• W1574672893 cites W2003481405 @default.
• W1574672893 cites W2004691998 @default.
• W1574672893 cites W2007055071 @default.
• W1574672893 cites W2009075135 @default.
• W1574672893 cites W2013390781 @default.
• W1574672893 cites W2020177273 @default.
• W1574672893 cites W2029056840 @default.
• W1574672893 cites W2032326371 @default.
• W1574672893 cites W2032748445 @default.
• W1574672893 cites W2038892288 @default.
• W1574672893 cites W2042386026 @default.
• W1574672893 cites W2049049823 @default.
• W1574672893 cites W2050844317 @default.
• W1574672893 cites W2063317846 @default.
• W1574672893 cites W2064467953 @default.
• W1574672893 cites W2065121279 @default.
• W1574672893 cites W2066798233 @default.
• W1574672893 cites W2067408128 @default.
• W1574672893 cites W2068332056 @default.
• W1574672893 cites W2070603332 @default.
• W1574672893 cites W2074231493 @default.
• W1574672893 cites W2086106892 @default.
• W1574672893 cites W2088516786 @default.
• W1574672893 cites W2102131116 @default.
• W1574672893 cites W2106558347 @default.
• W1574672893 cites W2110389243 @default.
• W1574672893 cites W2113964973 @default.
• W1574672893 cites W2120607615 @default.
• W1574672893 cites W2126273739 @default.
• W1574672893 cites W2128369415 @default.
• W1574672893 cites W2158129606 @default.
• W1574672893 cites W2180462291 @default.
• W1574672893 cites W2402446434 @default.
• W1574672893 cites W2410525911 @default.
• W1574672893 cites W2416111803 @default.
• W1574672893 cites W2468871828 @default.
• W1574672893 cites W49070171 @default.
• W1574672893 cites W68909338 @default.
• W1574672893 cites W7386683 @default.
• W1574672893 cites W2030858951 @default.
• W1574672893 doi "https://doi.org/10.1016/s0021-9258(19)37024-3" @default.
• W1574672893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1388157" @default.
• W1574672893 hasPublicationYear "1992" @default.
• W1574672893 type Work @default.
• W1574672893 sameAs 1574672893 @default.
• W1574672893 citedByCount "62" @default.
• W1574672893 countsByYear W15746728932013 @default.
• W1574672893 countsByYear W15746728932014 @default.
• W1574672893 countsByYear W15746728932015 @default.
• W1574672893 countsByYear W15746728932016 @default.
• W1574672893 countsByYear W15746728932018 @default.
• W1574672893 countsByYear W15746728932020 @default.
• W1574672893 countsByYear W15746728932022 @default.
• W1574672893 countsByYear W15746728932023 @default.
• W1574672893 crossrefType "journal-article" @default.
• W1574672893 hasAuthorship W1574672893A5002666918 @default.
• W1574672893 hasAuthorship W1574672893A5043994505 @default.
• W1574672893 hasAuthorship W1574672893A5044505827 @default.
• W1574672893 hasAuthorship W1574672893A5082786845 @default.

• next