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• W1574673988 abstract "ROLE OF THE TLM IN THE LOCALIZATION OF HRO-TWIST mRNA by Mehrin Farooq The subcellular localization of mRNA transcripts is critical in sorting cell fate determinants in the developing embryo of the glossiphoniid leech, Helodella robusta. Secondary structural motifs are recognized by /raws-acting factors which aid in the localization of these transported transcripts. Here, the Teloplasm Localization Motif (TLM), a secondary structural motif located in the 3'UTR of Hro-Twist, has been defined and characterized. The TLM comprises of a 20 unpaired nucleotide bulge, with one major and one minor stem loop structure projecting from it, and one stem structure in continuum with the rest of the mRNA 3'UTR. Site-directed mutagenesis was used to alter the TLM in four different ways: 1) converting the major stem loop structure into two smaller loops; 2) increasing the size of the major stem loop; 3) inserting a bulge in between the major and minor stem loops, and 4) distorting the structure completely. Three out of the four alterations in the TLM disrupted the polar localization of the HroTwist transcript indicating the crucial role of the TLM in its localization. Furthermore, structural modeling of other known leech transcripts that localize in the one-cell stage showed that three other mRNAs contain a similar motif (Lzf2 and Le-msx) raising the possibility that this structural element is important in the localization of a class of leech transcripts that localize early in the leech embryo. ACKNOWLEDGEMENTS I thank Dr. Julio Soto for giving me the opportunity to work on this project and guiding me every step of the way. I thank Dr. Miri Van Hoven and Dr. Robert Fowler for their advice. I thank my lab mates for the fun lab environment they provided and for their help in collecting leeches, especially Jonathan Choi who helped me many times when I was stuck. I thank Stephanie Mandal for helping me out with the figures for this thesis. I thank my family for their constant undying encouragement, my parents for their prayers, and especially my wonderful sisters Shahnaz, Mumtaz, Fowzia, and Mariam who have been my personal cheerleaders for the past three years. I thank Zainab Ismail for helping me when I most needed her. I thank Fazila Luqman for helping me to get started and never saying no when I asked for her help. I thank my sweet son, Omar, who always put things in perspective for me and because of whom the toughest days turned into the brightest ones. Finally I thank my husband, Muhammad Farooq, who has been my guiding force throughout this process and without whom this thesis would not have been possible." @default.
• W1574673988 created "2016-06-24" @default.
• W1574673988 creator A5012029056 @default.
• W1574673988 date "2019-05-03" @default.
• W1574673988 modified "2023-09-26" @default.
• W1574673988 title "Role of the TLM in the localization of HRO-TWIST mRNA" @default.
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