Matches in SemOpenAlex for { <https://semopenalex.org/work/W1574775008> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1574775008 endingPage "5286" @default.
- W1574775008 startingPage "5280" @default.
- W1574775008 abstract "Abstract Based on the results obtained in the previous paper (Liu, W.-K., Ascoli, M., and Ward, D. N. (1977) J. Biol. Chem. 252, 5274-5279) we have devised a method that permits the isolation of the subunits of ovine lutropin (oLH) in their form. The hormone was dissociated by guanidine HCl, and the subunits separated by salt precipitation (Sairam, M. R., and Li, C. H. (1974) Arch. Biochem. Biophys. 165, 709-714). The present method differs significantly from that of Sairam and Li in that all operations are carried out at pH 5.0 or higher, a condition we have shown to be crucial to maintain the integrity of the isolated subunits. The yield of subunits was 75 to 85%. The isolated subunits exceed 95% purity (i.e. contamination with the other subunit or intact lutropin is less than 5%), migrate as single bands in sodium dodecyl sulfate-polyacrylamide gels, and show only the NH2-terminal heterogeneity expected from that of the native hormone. Recombination of the subunits can be accomplished with 75 to 85% yield (with respect to mass) and with full recovery of biological activity." @default.
- W1574775008 created "2016-06-24" @default.
- W1574775008 creator A5057802739 @default.
- W1574775008 creator A5065877267 @default.
- W1574775008 creator A5089145622 @default.
- W1574775008 date "1977-08-01" @default.
- W1574775008 modified "2023-09-27" @default.
- W1574775008 title "Isolation and recombination of ovine lutropin subunits with complete recovery of biological activity." @default.
- W1574775008 cites W1482307610 @default.
- W1574775008 cites W1497874162 @default.
- W1574775008 cites W1525825852 @default.
- W1574775008 cites W1545385123 @default.
- W1574775008 cites W1551833234 @default.
- W1574775008 cites W1573609576 @default.
- W1574775008 cites W1579245994 @default.
- W1574775008 cites W1585532500 @default.
- W1574775008 cites W1589373559 @default.
- W1574775008 cites W1591714527 @default.
- W1574775008 cites W1593025965 @default.
- W1574775008 cites W1605926852 @default.
- W1574775008 cites W1627403859 @default.
- W1574775008 cites W1858643796 @default.
- W1574775008 cites W1964943989 @default.
- W1574775008 cites W1970099056 @default.
- W1574775008 cites W1978422280 @default.
- W1574775008 cites W1982715176 @default.
- W1574775008 cites W1993882032 @default.
- W1574775008 cites W2004479749 @default.
- W1574775008 cites W2026090921 @default.
- W1574775008 cites W2026771440 @default.
- W1574775008 cites W2032067145 @default.
- W1574775008 cites W2033765874 @default.
- W1574775008 cites W2034392218 @default.
- W1574775008 cites W2035391511 @default.
- W1574775008 cites W2048768181 @default.
- W1574775008 cites W2051475618 @default.
- W1574775008 cites W2055507679 @default.
- W1574775008 cites W2057470822 @default.
- W1574775008 cites W2065245877 @default.
- W1574775008 cites W2082088856 @default.
- W1574775008 cites W2088703956 @default.
- W1574775008 cites W2092246277 @default.
- W1574775008 cites W2093416481 @default.
- W1574775008 cites W2094556941 @default.
- W1574775008 cites W2100837269 @default.
- W1574775008 cites W2101417661 @default.
- W1574775008 cites W2229703472 @default.
- W1574775008 doi "https://doi.org/10.1016/s0021-9258(19)63344-2" @default.
- W1574775008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/885852" @default.
- W1574775008 hasPublicationYear "1977" @default.
- W1574775008 type Work @default.
- W1574775008 sameAs 1574775008 @default.
- W1574775008 citedByCount "28" @default.
- W1574775008 crossrefType "journal-article" @default.
- W1574775008 hasAuthorship W1574775008A5057802739 @default.
- W1574775008 hasAuthorship W1574775008A5065877267 @default.
- W1574775008 hasAuthorship W1574775008A5089145622 @default.
- W1574775008 hasBestOaLocation W15747750081 @default.
- W1574775008 hasConcept C104317684 @default.
- W1574775008 hasConcept C156695909 @default.
- W1574775008 hasConcept C185592680 @default.
- W1574775008 hasConcept C2775941552 @default.
- W1574775008 hasConcept C55493867 @default.
- W1574775008 hasConcept C60644358 @default.
- W1574775008 hasConcept C86803240 @default.
- W1574775008 hasConceptScore W1574775008C104317684 @default.
- W1574775008 hasConceptScore W1574775008C156695909 @default.
- W1574775008 hasConceptScore W1574775008C185592680 @default.
- W1574775008 hasConceptScore W1574775008C2775941552 @default.
- W1574775008 hasConceptScore W1574775008C55493867 @default.
- W1574775008 hasConceptScore W1574775008C60644358 @default.
- W1574775008 hasConceptScore W1574775008C86803240 @default.
- W1574775008 hasIssue "15" @default.
- W1574775008 hasLocation W15747750081 @default.
- W1574775008 hasOpenAccess W1574775008 @default.
- W1574775008 hasPrimaryLocation W15747750081 @default.
- W1574775008 hasRelatedWork W2018933503 @default.
- W1574775008 hasRelatedWork W2070232054 @default.
- W1574775008 hasRelatedWork W2089025702 @default.
- W1574775008 hasRelatedWork W2162223512 @default.
- W1574775008 hasRelatedWork W2291532526 @default.
- W1574775008 hasRelatedWork W2795192844 @default.
- W1574775008 hasRelatedWork W2899084033 @default.
- W1574775008 hasRelatedWork W2948807893 @default.
- W1574775008 hasRelatedWork W2992231355 @default.
- W1574775008 hasRelatedWork W2778153218 @default.
- W1574775008 hasVolume "252" @default.
- W1574775008 isParatext "false" @default.
- W1574775008 isRetracted "false" @default.
- W1574775008 magId "1574775008" @default.
- W1574775008 workType "article" @default.