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- W1575052873 abstract "This chapter focuses on the genetic complementation analysis of the interaction of hemolysin B (HlyB) with the hemolysin A (HlyA) signal sequence. A genetic approach is chosen because of the ease of genetic analysis in this bacterial system. The rapid generation time and the ability to screen large numbers of colonies by means of a plate-based hemolytic assay provide an opportunity to perform complex screens for complementing mutations of HlyB that compensate for the transport deficiency of HlyA signal mutations. The power of this approach allows studying the interaction of HlyB and the signal sequence of HlyA in detail than has generally been the case in studies of substrate recognition by other adenosine triphosphate-binding cassette (ABC) transporters. This genetic approach to identify interacting loci is by complementation, in which a phenotype caused by mutation of a specific region of one gene (signal sequence of HlyA) can be compensated for by mutations of the second gene (HlyB)." @default.
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- W1575052873 date "1998-01-01" @default.
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- W1575052873 title "[5] Structure—function analysis of hemolysin B" @default.
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- W1575052873 doi "https://doi.org/10.1016/s0076-6879(98)92007-9" @default.
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