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• W157518522 abstract "The advent of polymerase chain reaction (PCR) has greatly accelerated the progress of studies on the genomic structure of various organisms, and any region in even highly complex genomes can be specifically amplified in a few hours by the technique, if the flanking nucleotide sequences are known (Saiki et al., 1988). Virtually any DNA sequence can also be engineered by “copying and pasting” with PCR as a replacement for conventional recombinant DNA technology where DNA is manipulated by “cutting and pasting” using restriction endonucleases and ligase (Frohman and Martin, 1989). PCR therefore overcomes problems associated with the often limited availability of sites for the restriction endonucleases." @default.
• W157518522 created "2016-06-24" @default.
• W157518522 creator A5065512468 @default.
• W157518522 date "1994-01-01" @default.
• W157518522 modified "2023-09-24" @default.
• W157518522 title "Manipulation of DNA by PCR" @default.
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• W157518522 doi "https://doi.org/10.1007/978-1-4612-0257-8_1" @default.
• W157518522 hasPublicationYear "1994" @default.
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