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• W1575212562 abstract "Abstract In recent years, array‐based technologies, including DNA microarrays and protein‐detecting microarrays have been getting more attractive and promising as high‐throughput research/diagnostic tools. DNA microarrays comprise oligo‐DNA immobilized onto solid surfaces. Whereas protein‐detecting microarrays are categorized in several formats depending on classes of capturing agents that selectively bind to proteins of interest. Protein‐based capturing agents such as antibodies and fusion proteins are commonly immobilized onto solid surfaces. Alternatively, DNA/RNA aptamers, synthetic peptides, and small molecules including drugs and carbohydrates also act as capturing agents. In particular, peptides as capturing agents are very versatile, because they are chemically prepared according to the well‐established standard synthetic procedures and enable site‐specific and directed surface modifications. In this review, we describe the basic features of protein‐detecting microarray technologies (capturing agents, surface chemistry, and signal generation/readout); thereafter we suggest that the use of synthetic peptides is a very promising and powerful tool to monitor not only protein–peptide interactions but also enzymatic activities." @default.
• W1575212562 created "2016-06-24" @default.
• W1575212562 creator A5007273317 @default.
• W1575212562 creator A5037010022 @default.
• W1575212562 creator A5073861733 @default.
• W1575212562 date "2008-05-15" @default.
• W1575212562 modified "2023-10-10" @default.
• W1575212562 title "Proteins: Array‐Based Techniques" @default.
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