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• W1575481464 abstract "iii 1 General Introduction 1 1.1 The Innate and Adaptive Immune Systems 1 1.1.1 General Overview 1 1.1.2 The Innate Immune System 1 1.1.3 The Adaptive Immune System 3 1.1.4 Links between the innate & adaptive immune systems 5 1.2 The T-cell 8 1.2.1 The αβ TCR 8 1.2.2 TCR Diversity & Repertoire 15 1.2.3 The Major Histocompatibility Complex 20 1.3 T-cell subsets expressing invariant TCRα chains 28 1.3.1 Invariant NKT-cells and CD1d 28 1.3.2 MAIT Cells and MR1 30 1.4 Aims of the Project 40 2 Materials and Methods 41 2.1 Experimental animals 41 2.1.1 Post-mortem and collection of tissue samples 41 2.1.2 Cannulation of the efferent gastric lymph duct 41 2.2 Chemicals, solutions and plastic ware 42 2.3 Cellular techniques 42 2.3.1 Cell culture 42 2.3.2 Gene transfer 45 2.3.3 Expression analysis 46 2.4 Molecular techniques 50 2.4.1 Preparation of nucleic acids 50 2.4.2 Polymerase chain reactions 54 2.4.3 Analysis of nucleic acids 57 2.4.4 Cloning into plasmid vectors 61 2.4.5 Transformation and analysis of transformed E. coli 63 2.5 Sequence data and analysis 64 2.5.1 Sequence databases & search engines 64 2.5.2 Sequence analysis 65 2.5.3 TCR sequence nomenclature 65 3 Molecular Cloning and Characteristics of Ruminant MAIT 66 3." @default.
• W1575481464 created "2016-06-24" @default.
• W1575481464 creator A5055991097 @default.
• W1575481464 date "2010-01-01" @default.
• W1575481464 modified "2023-09-24" @default.
• W1575481464 title "Characterisation of mucosal associated invariant T-cells and MR1 in ruminants" @default.
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