Matches in SemOpenAlex for { <https://semopenalex.org/work/W1575638085> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W1575638085 endingPage "e1153" @default.
- W1575638085 startingPage "e1153" @default.
- W1575638085 abstract "The fungus Colletotrichum cereale incites anthracnose disease on Poa annua (annual bluegrass) turfgrass. Anthracnose disease is geographically widespread throughout the world and highly destructive to cool-season turfgrasses, with infections by C. cereale resulting in extensive turf loss. Comprehensive research aimed at controlling turfgrass anthracnose has been performed in the field, but knowledge of the causal organism and its basic biology is still needed. In particular, the lack of a reliable greenhouse-based inoculation protocol performed under controlled environmental conditions is an obstacle to the study of C. cereale and anthracnose disease. Our objective was to develop a consistent and reproducible inoculation protocol for the two major genetic lineages of C. cereale. By adapting previously successful field-based protocols and combining with components of existing inoculation procedures, the method we developed consistently produced C. cereale infection on two susceptible P. annua biotypes. Approximately 7 to 10 days post-inoculation, plants exhibited chlorosis and thinning consistent with anthracnose disease symptomology. Morphological inspection of inoculated plants revealed visual signs of the fungus (appressoria and acervuli), although acervuli were not always present. After stringent surface sterilization of inoculated host tissue, C. cereale was consistently re-isolated from symptomatic tissue. Real-time PCR detection analysis based on the Apn2 marker confirmed the presence of the pathogen in host tissue, with both lineages of C. cereale detected from all inoculated plants. When a humidifier was not used, no infection developed for any biotypes or fungal isolates tested. The inoculation protocol described here marks significant progress for in planta studies of C. cereale, and will enable scientifically reproducible investigations of the biology, infectivity and lifestyle of this important grass pathogen." @default.
- W1575638085 created "2016-06-24" @default.
- W1575638085 creator A5019598947 @default.
- W1575638085 creator A5020633846 @default.
- W1575638085 creator A5052353834 @default.
- W1575638085 creator A5075548435 @default.
- W1575638085 date "2015-08-13" @default.
- W1575638085 modified "2023-09-23" @default.
- W1575638085 title "Development of a greenhouse-based inoculation protocol for the fungus<i>Colletotrichum cereale</i>pathogenic to annual bluegrass (<i>Poa annua</i>)" @default.
- W1575638085 cites W1410920760 @default.
- W1575638085 cites W2001549591 @default.
- W1575638085 cites W2003266704 @default.
- W1575638085 cites W2010191191 @default.
- W1575638085 cites W2031980969 @default.
- W1575638085 cites W2039764499 @default.
- W1575638085 cites W2077490962 @default.
- W1575638085 cites W2079657006 @default.
- W1575638085 cites W2085258124 @default.
- W1575638085 cites W2095417386 @default.
- W1575638085 cites W2140782797 @default.
- W1575638085 cites W2145199617 @default.
- W1575638085 cites W2146554279 @default.
- W1575638085 cites W2154399010 @default.
- W1575638085 cites W2155748351 @default.
- W1575638085 cites W2163807437 @default.
- W1575638085 doi "https://doi.org/10.7717/peerj.1153" @default.
- W1575638085 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4558069" @default.
- W1575638085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26339538" @default.
- W1575638085 hasPublicationYear "2015" @default.
- W1575638085 type Work @default.
- W1575638085 sameAs 1575638085 @default.
- W1575638085 citedByCount "4" @default.
- W1575638085 countsByYear W15756380852017 @default.
- W1575638085 countsByYear W15756380852020 @default.
- W1575638085 countsByYear W15756380852022 @default.
- W1575638085 countsByYear W15756380852023 @default.
- W1575638085 crossrefType "journal-article" @default.
- W1575638085 hasAuthorship W1575638085A5019598947 @default.
- W1575638085 hasAuthorship W1575638085A5020633846 @default.
- W1575638085 hasAuthorship W1575638085A5052353834 @default.
- W1575638085 hasAuthorship W1575638085A5075548435 @default.
- W1575638085 hasBestOaLocation W15756380851 @default.
- W1575638085 hasConcept C126203033 @default.
- W1575638085 hasConcept C144027150 @default.
- W1575638085 hasConcept C180032290 @default.
- W1575638085 hasConcept C2778987702 @default.
- W1575638085 hasConcept C2779678110 @default.
- W1575638085 hasConcept C2780354985 @default.
- W1575638085 hasConcept C46757340 @default.
- W1575638085 hasConcept C59822182 @default.
- W1575638085 hasConcept C74600772 @default.
- W1575638085 hasConcept C86803240 @default.
- W1575638085 hasConceptScore W1575638085C126203033 @default.
- W1575638085 hasConceptScore W1575638085C144027150 @default.
- W1575638085 hasConceptScore W1575638085C180032290 @default.
- W1575638085 hasConceptScore W1575638085C2778987702 @default.
- W1575638085 hasConceptScore W1575638085C2779678110 @default.
- W1575638085 hasConceptScore W1575638085C2780354985 @default.
- W1575638085 hasConceptScore W1575638085C46757340 @default.
- W1575638085 hasConceptScore W1575638085C59822182 @default.
- W1575638085 hasConceptScore W1575638085C74600772 @default.
- W1575638085 hasConceptScore W1575638085C86803240 @default.
- W1575638085 hasLocation W15756380851 @default.
- W1575638085 hasLocation W15756380852 @default.
- W1575638085 hasLocation W15756380853 @default.
- W1575638085 hasLocation W15756380854 @default.
- W1575638085 hasLocation W15756380855 @default.
- W1575638085 hasOpenAccess W1575638085 @default.
- W1575638085 hasPrimaryLocation W15756380851 @default.
- W1575638085 hasRelatedWork W1575638085 @default.
- W1575638085 hasRelatedWork W2029181636 @default.
- W1575638085 hasRelatedWork W2050680630 @default.
- W1575638085 hasRelatedWork W2051186995 @default.
- W1575638085 hasRelatedWork W2053102627 @default.
- W1575638085 hasRelatedWork W2079686184 @default.
- W1575638085 hasRelatedWork W2115148694 @default.
- W1575638085 hasRelatedWork W23134534 @default.
- W1575638085 hasRelatedWork W2529738607 @default.
- W1575638085 hasRelatedWork W3213707308 @default.
- W1575638085 hasVolume "3" @default.
- W1575638085 isParatext "false" @default.
- W1575638085 isRetracted "false" @default.
- W1575638085 magId "1575638085" @default.
- W1575638085 workType "article" @default.