Matches in SemOpenAlex for { <https://semopenalex.org/work/W1576377989> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1576377989 endingPage "6965" @default.
- W1576377989 startingPage "6960" @default.
- W1576377989 abstract "The characteristics of yeast eukaryotic initiation factor 2 (eIF-2) and Co-eIF-2A have been studied and compared with those of the corresponding factors from rabbit reticulocytes. 1) Unlike eIF-2r, purified eIF-2y did not contain bound GDP. 2) Purified eIF-2y preparation contained GTPase activity and dephosphorylated GTP to GDP. 3) An anti-eIF-2r preparation which predominantly precipitated the gamma-subunit (Mr 54,000) of eIF-2r also precipitated the larger subunit (Mr 54,000) of eIF-2y. 4) Unlike eIF-2r, ternary complex formation by eIF-2y was not inhibited by Mg2+. 5) Both Co-eIF-2A20y and Co-eIF-2r significantly enhanced Met-tRNAf binding to eIF-2y and, again, Mg2+ did not have any effect on this stimulated Met-tRNAf binding to eIF-2y. 6) Both Co-eIF-2A20y and Co-eIF-2r were similarly effective in stimulating Met-tRNAf binding to eIF-2r in the absence of Mg2+. However, in the presence of Mg2+, Co-eIF-2A20y was significantly less effective than Co-eIF-2r as Co-eIF-2A20y did not promote displacement of GDP from eIF-2r X GDP. 7) eIF-2y bound [3H]GDP and this binding was significantly enhanced in the presence of Mg2+. Also, [3H]GDP in the preformed eIF-2y X [3H]GDP complex was rapidly exchanged with exogenously added unlabeled GDP in the presence of Mg2+. Co-eIF-2A20y had no effect on GDP binding to eIF-2y nor on GDP exchange reactions. 8) Reticulocyte heme-regulated protein synthesis inhibitor, which phosphorylated almost completely (in excess of 80%) the alpha-subunit (Mr 38,000) of eIF-2r, also phosphorylated similarly the smaller subunit (Mr 36,000) of eIF-2y. However, such phosphorylation had no significant effect on ternary complex formation, GDP binding, and GDP exchange reactions." @default.
- W1576377989 created "2016-06-24" @default.
- W1576377989 creator A5027884511 @default.
- W1576377989 creator A5046784225 @default.
- W1576377989 creator A5053790031 @default.
- W1576377989 creator A5076507508 @default.
- W1576377989 creator A5080073796 @default.
- W1576377989 date "1985-06-01" @default.
- W1576377989 modified "2023-09-26" @default.
- W1576377989 title "A comparative study of the characteristics of eIF-2 and eIF-2-ancillary factor activities from yeast Saccharomyces cerevisiae and rabbit reticulocytes." @default.
- W1576377989 cites W1480406908 @default.
- W1576377989 cites W1481139836 @default.
- W1576377989 cites W1515049367 @default.
- W1576377989 cites W1520635903 @default.
- W1576377989 cites W1527791774 @default.
- W1576377989 cites W1548251237 @default.
- W1576377989 cites W1551930510 @default.
- W1576377989 cites W1555432182 @default.
- W1576377989 cites W1557409411 @default.
- W1576377989 cites W1558494944 @default.
- W1576377989 cites W1566160724 @default.
- W1576377989 cites W1576152125 @default.
- W1576377989 cites W1600280031 @default.
- W1576377989 cites W1968817562 @default.
- W1576377989 cites W1970081885 @default.
- W1576377989 cites W1970529107 @default.
- W1576377989 cites W1970736180 @default.
- W1576377989 cites W1990344643 @default.
- W1576377989 cites W1992910367 @default.
- W1576377989 cites W1993932155 @default.
- W1576377989 cites W2015901124 @default.
- W1576377989 cites W2047662834 @default.
- W1576377989 cites W2057635096 @default.
- W1576377989 cites W2079503122 @default.
- W1576377989 cites W2144147714 @default.
- W1576377989 cites W258376682 @default.
- W1576377989 cites W3022737785 @default.
- W1576377989 cites W4234038697 @default.
- W1576377989 cites W4300715019 @default.
- W1576377989 cites W72118176 @default.
- W1576377989 doi "https://doi.org/10.1016/s0021-9258(18)88874-3" @default.
- W1576377989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3888990" @default.
- W1576377989 hasPublicationYear "1985" @default.
- W1576377989 type Work @default.
- W1576377989 sameAs 1576377989 @default.
- W1576377989 citedByCount "18" @default.
- W1576377989 crossrefType "journal-article" @default.
- W1576377989 hasAuthorship W1576377989A5027884511 @default.
- W1576377989 hasAuthorship W1576377989A5046784225 @default.
- W1576377989 hasAuthorship W1576377989A5053790031 @default.
- W1576377989 hasAuthorship W1576377989A5076507508 @default.
- W1576377989 hasAuthorship W1576377989A5080073796 @default.
- W1576377989 hasBestOaLocation W15763779891 @default.
- W1576377989 hasConcept C185592680 @default.
- W1576377989 hasConcept C2777576037 @default.
- W1576377989 hasConcept C2779222958 @default.
- W1576377989 hasConcept C2779884254 @default.
- W1576377989 hasConcept C38652104 @default.
- W1576377989 hasConcept C41008148 @default.
- W1576377989 hasConcept C55493867 @default.
- W1576377989 hasConcept C86803240 @default.
- W1576377989 hasConceptScore W1576377989C185592680 @default.
- W1576377989 hasConceptScore W1576377989C2777576037 @default.
- W1576377989 hasConceptScore W1576377989C2779222958 @default.
- W1576377989 hasConceptScore W1576377989C2779884254 @default.
- W1576377989 hasConceptScore W1576377989C38652104 @default.
- W1576377989 hasConceptScore W1576377989C41008148 @default.
- W1576377989 hasConceptScore W1576377989C55493867 @default.
- W1576377989 hasConceptScore W1576377989C86803240 @default.
- W1576377989 hasIssue "11" @default.
- W1576377989 hasLocation W15763779891 @default.
- W1576377989 hasOpenAccess W1576377989 @default.
- W1576377989 hasPrimaryLocation W15763779891 @default.
- W1576377989 hasRelatedWork W2009810460 @default.
- W1576377989 hasRelatedWork W2025917340 @default.
- W1576377989 hasRelatedWork W2026244176 @default.
- W1576377989 hasRelatedWork W2032533427 @default.
- W1576377989 hasRelatedWork W2062385823 @default.
- W1576377989 hasRelatedWork W2078693732 @default.
- W1576377989 hasRelatedWork W2087346710 @default.
- W1576377989 hasRelatedWork W2242477415 @default.
- W1576377989 hasRelatedWork W2343067289 @default.
- W1576377989 hasRelatedWork W2606340556 @default.
- W1576377989 hasVolume "260" @default.
- W1576377989 isParatext "false" @default.
- W1576377989 isRetracted "false" @default.
- W1576377989 magId "1576377989" @default.
- W1576377989 workType "article" @default.