Matches in SemOpenAlex for { <https://semopenalex.org/work/W1576416579> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1576416579 endingPage "100" @default.
- W1576416579 startingPage "91" @default.
- W1576416579 abstract "Reactive oxygen species (ROS) such as superoxide (O2–) hydrogen peroxide (H2O2), and nitric oxide (NO), generated by activated phagocytes, are crucial for the immune response against pathogens. It has become evident that ROS, while responsible for indiscriminate oxidative cell damage when released in excess amounts, can also, at low concentrations and in very limited time frames, behave as fine modulators of important intracellular events, such as protein phosphorylation and gene transcription, through which the cell response to environmental stimuli is triggered. In view of these considerations, a protocol in which the intracellular production of oxygen species grown at different plating densities is evaluated in single cells by flow cytometry after cell loading with the peroxide-sensitive probe dichlorofluorescein diacetate (DCF-DA) and following rapid detachment from the substrate. A flow chart summarizing the basic protocol for determination of intracellular ROS at different plating densities is also presented. A method to evaluate the intracellular content of oxidants as a function of cell density—that is, in cells plated at different degree of confluence is developed. The method is based on the cytofluorimetric analysis of the oxidative modification of a widely used probe, dichlorofluorescein diacetate that gains a bright green fluorescence on interaction with hydrogen peroxide and organic peroxides." @default.
- W1576416579 created "2016-06-24" @default.
- W1576416579 creator A5011249978 @default.
- W1576416579 creator A5023386989 @default.
- W1576416579 creator A5025609863 @default.
- W1576416579 creator A5026476548 @default.
- W1576416579 creator A5031084295 @default.
- W1576416579 creator A5050578320 @default.
- W1576416579 date "2002-01-01" @default.
- W1576416579 modified "2023-10-15" @default.
- W1576416579 title "[8] Determination of intracellular reactive oxygen species as function of cell density" @default.
- W1576416579 cites W1964345623 @default.
- W1576416579 cites W1968443660 @default.
- W1576416579 cites W1970405699 @default.
- W1576416579 cites W1979971695 @default.
- W1576416579 cites W1999754601 @default.
- W1576416579 cites W2001300582 @default.
- W1576416579 cites W2009588836 @default.
- W1576416579 cites W2011272696 @default.
- W1576416579 cites W2011574071 @default.
- W1576416579 cites W2020138346 @default.
- W1576416579 cites W2048569323 @default.
- W1576416579 cites W2049850860 @default.
- W1576416579 cites W2056135909 @default.
- W1576416579 cites W2059459858 @default.
- W1576416579 cites W2072727557 @default.
- W1576416579 cites W2077104885 @default.
- W1576416579 cites W2081629504 @default.
- W1576416579 cites W2081748503 @default.
- W1576416579 cites W2109662937 @default.
- W1576416579 cites W2127744067 @default.
- W1576416579 cites W2183191437 @default.
- W1576416579 cites W4246915719 @default.
- W1576416579 doi "https://doi.org/10.1016/s0076-6879(02)52010-3" @default.
- W1576416579 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12125380" @default.
- W1576416579 hasPublicationYear "2002" @default.
- W1576416579 type Work @default.
- W1576416579 sameAs 1576416579 @default.
- W1576416579 citedByCount "13" @default.
- W1576416579 countsByYear W15764165792020 @default.
- W1576416579 countsByYear W15764165792021 @default.
- W1576416579 crossrefType "book-chapter" @default.
- W1576416579 hasAuthorship W1576416579A5011249978 @default.
- W1576416579 hasAuthorship W1576416579A5023386989 @default.
- W1576416579 hasAuthorship W1576416579A5025609863 @default.
- W1576416579 hasAuthorship W1576416579A5026476548 @default.
- W1576416579 hasAuthorship W1576416579A5031084295 @default.
- W1576416579 hasAuthorship W1576416579A5050578320 @default.
- W1576416579 hasConcept C12554922 @default.
- W1576416579 hasConcept C153911025 @default.
- W1576416579 hasConcept C178790620 @default.
- W1576416579 hasConcept C181199279 @default.
- W1576416579 hasConcept C185592680 @default.
- W1576416579 hasConcept C2779964046 @default.
- W1576416579 hasConcept C2780795997 @default.
- W1576416579 hasConcept C48349386 @default.
- W1576416579 hasConcept C519581460 @default.
- W1576416579 hasConcept C533411734 @default.
- W1576416579 hasConcept C553184892 @default.
- W1576416579 hasConcept C55493867 @default.
- W1576416579 hasConcept C57600042 @default.
- W1576416579 hasConcept C79879829 @default.
- W1576416579 hasConcept C86803240 @default.
- W1576416579 hasConcept C95444343 @default.
- W1576416579 hasConceptScore W1576416579C12554922 @default.
- W1576416579 hasConceptScore W1576416579C153911025 @default.
- W1576416579 hasConceptScore W1576416579C178790620 @default.
- W1576416579 hasConceptScore W1576416579C181199279 @default.
- W1576416579 hasConceptScore W1576416579C185592680 @default.
- W1576416579 hasConceptScore W1576416579C2779964046 @default.
- W1576416579 hasConceptScore W1576416579C2780795997 @default.
- W1576416579 hasConceptScore W1576416579C48349386 @default.
- W1576416579 hasConceptScore W1576416579C519581460 @default.
- W1576416579 hasConceptScore W1576416579C533411734 @default.
- W1576416579 hasConceptScore W1576416579C553184892 @default.
- W1576416579 hasConceptScore W1576416579C55493867 @default.
- W1576416579 hasConceptScore W1576416579C57600042 @default.
- W1576416579 hasConceptScore W1576416579C79879829 @default.
- W1576416579 hasConceptScore W1576416579C86803240 @default.
- W1576416579 hasConceptScore W1576416579C95444343 @default.
- W1576416579 hasLocation W15764165791 @default.
- W1576416579 hasLocation W15764165792 @default.
- W1576416579 hasOpenAccess W1576416579 @default.
- W1576416579 hasPrimaryLocation W15764165791 @default.
- W1576416579 hasRelatedWork W1984052653 @default.
- W1576416579 hasRelatedWork W2014112090 @default.
- W1576416579 hasRelatedWork W2019421902 @default.
- W1576416579 hasRelatedWork W2108695382 @default.
- W1576416579 hasRelatedWork W2149286456 @default.
- W1576416579 hasRelatedWork W2350811517 @default.
- W1576416579 hasRelatedWork W2998345054 @default.
- W1576416579 hasRelatedWork W2999317767 @default.
- W1576416579 hasRelatedWork W4239785121 @default.
- W1576416579 hasRelatedWork W53828686 @default.
- W1576416579 isParatext "false" @default.
- W1576416579 isRetracted "false" @default.
- W1576416579 magId "1576416579" @default.
- W1576416579 workType "book-chapter" @default.