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- W1576492698 abstract "Publisher Summary This chapter focuses on cloning zebrafish by nuclear transfer. Cloning of zebrafish by nuclear transfer is a complex procedure involving cell culture, egg selection, and micromanipulation of the eggs and cells, each step affecting the overall efficiency of cloning. The chapter provides a detailed description of the nuclear transfer procedure developed in laboratory. For nuclear transfer, long-term cultured cells are subjected to serum starvation by culturing them in DMEM medium supplemented with 0.5% FBS for 4 days and are then dissociated with trypsin/EDTA (0.25% trypsin/1 mM EDTA in PBS), washed once with PBS, and centrifuged at 1000 rpm for 5 min. Cells are suspended in DMEM containing 0.5% FBS and kept on ice until nuclear transfer. During nuclear transfer, both the recipient eggs and donor cells are placed into an inverted cover of a Falcon Tissue culture dish. The eggs are kept in a large drop of precooled Hank's solution supplemented with 1.5% BSA, and the donor cells are kept nearby in a drop of precooled DMEM medium supplemented with 0.5% FBS (v/v). Cloned zebrafish can be excellent models for studying effects of cloning on animal development. Developmental abnormalities in cloned zebrafish can be easily found because of in vitro and transparent embryogenesis. Short generation time and easy access to a large number of progenies mean that the effects of cloning can be monitored thoroughly in multiple generations in a relatively short time." @default.
- W1576492698 created "2016-06-24" @default.
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- W1576492698 date "2004-01-01" @default.
- W1576492698 modified "2023-10-16" @default.
- W1576492698 title "Cloning Zebrafish by Nuclear Transfer" @default.
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- W1576492698 doi "https://doi.org/10.1016/s0091-679x(04)77022-3" @default.
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