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- W1576514130 abstract "A multi-laboratory evaluation of high-performance liquid chromatographic methods for folic acid (FA) applied to several cereal samples revealed considerable variability in the results. It was found that laboratories that employed simple aqueous extraction techniques obtained FA values closer to values claimed on the food labels. An alkaline pH of 11 favored the dissolution of FA from sample matrices and yielded results that were in close agreement with values obtained by liquid chromatography–tandem mass spectrometry for the same samples. Aqueous extraction at pH 11 yielded FA values for 10 samples that were statistically not different from those determined by stable isotope dilution analyses. Some samples showed anomalous and high FA values on extraction at alkaline pH but this was not observed on extraction at pH 7.85. The use of α-amylase and protease, enzymes traditionally applied for sample preparation, did not provide advantages in the analysis of added folic acid in cereals samples." @default.
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- W1576514130 date "2011-03-18" @default.
- W1576514130 modified "2023-09-27" @default.
- W1576514130 title "Optimization of HPLC Methods for Analyzing Added Folic Acid in Fortified Foods" @default.
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- W1576514130 doi "https://doi.org/10.1002/9783527634156.ch10" @default.
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