Matches in SemOpenAlex for { <https://semopenalex.org/work/W1576672105> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W1576672105 endingPage "204" @default.
- W1576672105 startingPage "202" @default.
- W1576672105 abstract "Procedures for differentiation between wild and vaccine-derived strains of poliovirus are required, particularly in countries where wild and vaccine-related strains coexist. For this differentiation, we tested the method of Inouye and Hondo (S. Inouye and R. Hondo, Arch. Virol. 129:311-316, 1993) for discrimination of closely related viruses by using stringent microplate hybridization of PCR products. We used a pair of primers with enterovirus common sequences (between these primers there is a variable region for capsid proteins) for PCR using templates from wild and vaccine-derived poliovirus strains which were isolated in tissue culture and serotyped by neutralization assay. We also used the same primers for preparation of probes, which were labelled by incorporation of biotin-dUTP in the PCR, with the three original Sabin vaccine virus strains used as templates. The amplified DNAs from the isolates were immobilized on microplate wells and were then hybridized with the labelled probes. We found that, under the usual hybridization conditions, the Sabin vaccine virus strain probes hybridized with both wild and vaccine-derived viruses, but under stringent conditions, they reacted only with vaccine-derived viruses of the same serotype, clearly differentiating these from wild-type viruses." @default.
- W1576672105 created "2016-06-24" @default.
- W1576672105 creator A5007400491 @default.
- W1576672105 creator A5022274918 @default.
- W1576672105 creator A5043548470 @default.
- W1576672105 creator A5050812125 @default.
- W1576672105 creator A5056624276 @default.
- W1576672105 creator A5074888588 @default.
- W1576672105 date "1994-01-01" @default.
- W1576672105 modified "2023-09-27" @default.
- W1576672105 title "Differentiation between wild and vaccine-derived strains of poliovirus by stringent microplate hybridization of PCR products" @default.
- W1576672105 cites W1570828652 @default.
- W1576672105 cites W1946251729 @default.
- W1576672105 cites W1965447103 @default.
- W1576672105 cites W1985970112 @default.
- W1576672105 cites W2022291864 @default.
- W1576672105 cites W2034544505 @default.
- W1576672105 cites W2037083673 @default.
- W1576672105 cites W2038998018 @default.
- W1576672105 cites W2041578595 @default.
- W1576672105 cites W2058423707 @default.
- W1576672105 cites W2156949167 @default.
- W1576672105 cites W2157707773 @default.
- W1576672105 doi "https://doi.org/10.1128/jcm.32.1.202-204.1994" @default.
- W1576672105 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/262996" @default.
- W1576672105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8126180" @default.
- W1576672105 hasPublicationYear "1994" @default.
- W1576672105 type Work @default.
- W1576672105 sameAs 1576672105 @default.
- W1576672105 citedByCount "19" @default.
- W1576672105 countsByYear W15766721052012 @default.
- W1576672105 countsByYear W15766721052013 @default.
- W1576672105 countsByYear W15766721052019 @default.
- W1576672105 crossrefType "journal-article" @default.
- W1576672105 hasAuthorship W1576672105A5007400491 @default.
- W1576672105 hasAuthorship W1576672105A5022274918 @default.
- W1576672105 hasAuthorship W1576672105A5043548470 @default.
- W1576672105 hasAuthorship W1576672105A5050812125 @default.
- W1576672105 hasAuthorship W1576672105A5056624276 @default.
- W1576672105 hasAuthorship W1576672105A5074888588 @default.
- W1576672105 hasBestOaLocation W15766721051 @default.
- W1576672105 hasConcept C10389963 @default.
- W1576672105 hasConcept C153911025 @default.
- W1576672105 hasConcept C159047783 @default.
- W1576672105 hasConcept C202878990 @default.
- W1576672105 hasConcept C2522874641 @default.
- W1576672105 hasConcept C2777888124 @default.
- W1576672105 hasConcept C2779631682 @default.
- W1576672105 hasConcept C86803240 @default.
- W1576672105 hasConcept C89423630 @default.
- W1576672105 hasConceptScore W1576672105C10389963 @default.
- W1576672105 hasConceptScore W1576672105C153911025 @default.
- W1576672105 hasConceptScore W1576672105C159047783 @default.
- W1576672105 hasConceptScore W1576672105C202878990 @default.
- W1576672105 hasConceptScore W1576672105C2522874641 @default.
- W1576672105 hasConceptScore W1576672105C2777888124 @default.
- W1576672105 hasConceptScore W1576672105C2779631682 @default.
- W1576672105 hasConceptScore W1576672105C86803240 @default.
- W1576672105 hasConceptScore W1576672105C89423630 @default.
- W1576672105 hasIssue "1" @default.
- W1576672105 hasLocation W15766721051 @default.
- W1576672105 hasLocation W15766721052 @default.
- W1576672105 hasOpenAccess W1576672105 @default.
- W1576672105 hasPrimaryLocation W15766721051 @default.
- W1576672105 hasRelatedWork W111910891 @default.
- W1576672105 hasRelatedWork W2006840513 @default.
- W1576672105 hasRelatedWork W2013921798 @default.
- W1576672105 hasRelatedWork W2059616773 @default.
- W1576672105 hasRelatedWork W2064399553 @default.
- W1576672105 hasRelatedWork W2111210021 @default.
- W1576672105 hasRelatedWork W2161186322 @default.
- W1576672105 hasRelatedWork W2230912919 @default.
- W1576672105 hasRelatedWork W2370850119 @default.
- W1576672105 hasRelatedWork W4292757077 @default.
- W1576672105 hasVolume "32" @default.
- W1576672105 isParatext "false" @default.
- W1576672105 isRetracted "false" @default.
- W1576672105 magId "1576672105" @default.
- W1576672105 workType "article" @default.