Matches in SemOpenAlex for { <https://semopenalex.org/work/W1576686733> ?p ?o ?g. }
- W1576686733 endingPage "24321" @default.
- W1576686733 startingPage "24315" @default.
- W1576686733 abstract "Ubiquitin-activating enzyme, E1, is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates and represents a potential target for regulation in the metabolic control of the conjugation reaction. Antiserum raised against human E1 recognizes two immunoreactive proteins in extracts from several human cell lines and animal tissues. We have characterized these two immunoreactive proteins in HeLa cells and present evidence that they are isoforms of E1. We have designated these isoforms as E1(110 kDa) and E1(117 kDa) to reflect their apparent molecular masses determined from SDS-polyacrylamide gel electrophoresis. These two immunoreactive proteins are immunologically similar, have nearly identical peptide maps, and comigrate with enzymatic activity characteristic of E1 in native polyacrylamide gel electrophoretic separations. Pulse-labeling experiments reveal that both isoforms are long-lived in vivo with degradation rates which are inconsistent with a proenzyme/enzyme model. Furthermore, their rates of degradation, which vary depending on the cell line studied, are kinetically distinguishable in contact-inhibited human lung fibroblasts. This work represents the first demonstration of E1 isoforms in a non-plant species and carries important implications for studies of the regulatory mechanisms controlling ubiquitin conjugation." @default.
- W1576686733 created "2016-06-24" @default.
- W1576686733 creator A5002087699 @default.
- W1576686733 creator A5014446674 @default.
- W1576686733 date "1992-12-01" @default.
- W1576686733 modified "2023-09-30" @default.
- W1576686733 title "Isoforms of mammalian ubiquitin-activating enzyme." @default.
- W1576686733 cites W135235169 @default.
- W1576686733 cites W1483766000 @default.
- W1576686733 cites W1487877558 @default.
- W1576686733 cites W1489931243 @default.
- W1576686733 cites W1493071013 @default.
- W1576686733 cites W1494460922 @default.
- W1576686733 cites W1523951968 @default.
- W1576686733 cites W1526095797 @default.
- W1576686733 cites W1532738146 @default.
- W1576686733 cites W1539814399 @default.
- W1576686733 cites W1549578642 @default.
- W1576686733 cites W1586828670 @default.
- W1576686733 cites W1592652234 @default.
- W1576686733 cites W1607209090 @default.
- W1576686733 cites W1628512331 @default.
- W1576686733 cites W1964750756 @default.
- W1576686733 cites W1964822816 @default.
- W1576686733 cites W1968229731 @default.
- W1576686733 cites W1978186903 @default.
- W1576686733 cites W1979408355 @default.
- W1576686733 cites W1985632088 @default.
- W1576686733 cites W1990916634 @default.
- W1576686733 cites W1991101546 @default.
- W1576686733 cites W2001313857 @default.
- W1576686733 cites W2008689421 @default.
- W1576686733 cites W2011147958 @default.
- W1576686733 cites W2013289317 @default.
- W1576686733 cites W2045121112 @default.
- W1576686733 cites W2048716882 @default.
- W1576686733 cites W2058852380 @default.
- W1576686733 cites W2059779415 @default.
- W1576686733 cites W2062617918 @default.
- W1576686733 cites W2072389679 @default.
- W1576686733 cites W2073946289 @default.
- W1576686733 cites W2077297615 @default.
- W1576686733 cites W2082986659 @default.
- W1576686733 cites W2083364752 @default.
- W1576686733 cites W2089728262 @default.
- W1576686733 cites W2090594597 @default.
- W1576686733 cites W2090979749 @default.
- W1576686733 cites W2091676399 @default.
- W1576686733 cites W2094919481 @default.
- W1576686733 cites W2100837269 @default.
- W1576686733 cites W2101108802 @default.
- W1576686733 cites W2111817213 @default.
- W1576686733 cites W2140182533 @default.
- W1576686733 cites W2146437721 @default.
- W1576686733 cites W2169996826 @default.
- W1576686733 cites W2203544760 @default.
- W1576686733 cites W4251683570 @default.
- W1576686733 cites W859618145 @default.
- W1576686733 doi "https://doi.org/10.1016/s0021-9258(18)35767-3" @default.
- W1576686733 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1447181" @default.
- W1576686733 hasPublicationYear "1992" @default.
- W1576686733 type Work @default.
- W1576686733 sameAs 1576686733 @default.
- W1576686733 citedByCount "31" @default.
- W1576686733 countsByYear W15766867332015 @default.
- W1576686733 countsByYear W15766867332020 @default.
- W1576686733 countsByYear W15766867332021 @default.
- W1576686733 countsByYear W15766867332022 @default.
- W1576686733 crossrefType "journal-article" @default.
- W1576686733 hasAuthorship W1576686733A5002087699 @default.
- W1576686733 hasAuthorship W1576686733A5014446674 @default.
- W1576686733 hasBestOaLocation W15766867331 @default.
- W1576686733 hasConcept C104317684 @default.
- W1576686733 hasConcept C181199279 @default.
- W1576686733 hasConcept C185592680 @default.
- W1576686733 hasConcept C25602115 @default.
- W1576686733 hasConcept C53345823 @default.
- W1576686733 hasConcept C55493867 @default.
- W1576686733 hasConcept C86803240 @default.
- W1576686733 hasConcept C95444343 @default.
- W1576686733 hasConceptScore W1576686733C104317684 @default.
- W1576686733 hasConceptScore W1576686733C181199279 @default.
- W1576686733 hasConceptScore W1576686733C185592680 @default.
- W1576686733 hasConceptScore W1576686733C25602115 @default.
- W1576686733 hasConceptScore W1576686733C53345823 @default.
- W1576686733 hasConceptScore W1576686733C55493867 @default.
- W1576686733 hasConceptScore W1576686733C86803240 @default.
- W1576686733 hasConceptScore W1576686733C95444343 @default.
- W1576686733 hasIssue "34" @default.
- W1576686733 hasLocation W15766867331 @default.
- W1576686733 hasOpenAccess W1576686733 @default.
- W1576686733 hasPrimaryLocation W15766867331 @default.
- W1576686733 hasRelatedWork W1573247099 @default.
- W1576686733 hasRelatedWork W1969633098 @default.
- W1576686733 hasRelatedWork W2025398173 @default.
- W1576686733 hasRelatedWork W2033084145 @default.
- W1576686733 hasRelatedWork W2095693152 @default.
- W1576686733 hasRelatedWork W2809186646 @default.