Matches in SemOpenAlex for { <https://semopenalex.org/work/W1576964650> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1576964650 endingPage "128" @default.
- W1576964650 startingPage "121" @default.
- W1576964650 abstract "A 0.7-kb DNA fragment, amplified by the randomly amplified polymorphic DNA (RAPD) method from Ralstonia solanacearum total DNA, was cloned and evaluated as a specific DNA probe. This 0.7-kb DNA fragment hybridized to a 2.7-kb EcoRI fragment in the EcoRI-digested total DNA of R. solanacearum. The 2.7-kb EcoRI fragment was also cloned and hybridized only to R. solanacearum but not to other Pseudomonas spp. pathovars of Xanthomonas campestris, and Erwinia spp. tested. The DNA sequence of this 2.7-kb fragment was obtained and used to design specific oligonucleotide primers for polymerase chain reaction (PCR) amplification. The primers amplified the same 1.1-kb PCR product from all R. solanacearum strains tested and failed to amplify DNA from any other plant pathogenic bacterial strains tested. DNA isolated from several saprophytic bacteria did not produce any PCR products with these primers. This specific PCR for R. solanacearum was also performed from colonies grown on BG medium with similar results. The sensitivity of the PCR assay using the specific primers was about 20 cells. The PCR assay was used to detect R. solanacearum in soil using these primer sets. No PCR product could be found when soil extract containing R. solanacea rum was used directly in the assay. DNA extraction from soil was needed for the success of PCR assay. A simple method for DNA extraction from soil for PCR assay was developed and can hasten the detection of R. solanacearum in soil." @default.
- W1576964650 created "2016-06-24" @default.
- W1576964650 creator A5035941395 @default.
- W1576964650 creator A5077043341 @default.
- W1576964650 date "2000-04-01" @default.
- W1576964650 modified "2023-09-24" @default.
- W1576964650 title "The design of specific primers for the detection of Ralstonia solanacearum in soil samples by polymerase chain reaction." @default.
- W1576964650 doi "https://doi.org/10.7016/bbas.200004.0121" @default.
- W1576964650 hasPublicationYear "2000" @default.
- W1576964650 type Work @default.
- W1576964650 sameAs 1576964650 @default.
- W1576964650 citedByCount "10" @default.
- W1576964650 countsByYear W15769646502014 @default.
- W1576964650 countsByYear W15769646502015 @default.
- W1576964650 crossrefType "journal-article" @default.
- W1576964650 hasAuthorship W1576964650A5035941395 @default.
- W1576964650 hasAuthorship W1576964650A5077043341 @default.
- W1576964650 hasConcept C104317684 @default.
- W1576964650 hasConcept C130123407 @default.
- W1576964650 hasConcept C153911025 @default.
- W1576964650 hasConcept C178790620 @default.
- W1576964650 hasConcept C185592680 @default.
- W1576964650 hasConcept C22744801 @default.
- W1576964650 hasConcept C2776460866 @default.
- W1576964650 hasConcept C2777563447 @default.
- W1576964650 hasConcept C2780675535 @default.
- W1576964650 hasConcept C49105822 @default.
- W1576964650 hasConcept C54355233 @default.
- W1576964650 hasConcept C552990157 @default.
- W1576964650 hasConcept C78063203 @default.
- W1576964650 hasConcept C86803240 @default.
- W1576964650 hasConcept C89423630 @default.
- W1576964650 hasConceptScore W1576964650C104317684 @default.
- W1576964650 hasConceptScore W1576964650C130123407 @default.
- W1576964650 hasConceptScore W1576964650C153911025 @default.
- W1576964650 hasConceptScore W1576964650C178790620 @default.
- W1576964650 hasConceptScore W1576964650C185592680 @default.
- W1576964650 hasConceptScore W1576964650C22744801 @default.
- W1576964650 hasConceptScore W1576964650C2776460866 @default.
- W1576964650 hasConceptScore W1576964650C2777563447 @default.
- W1576964650 hasConceptScore W1576964650C2780675535 @default.
- W1576964650 hasConceptScore W1576964650C49105822 @default.
- W1576964650 hasConceptScore W1576964650C54355233 @default.
- W1576964650 hasConceptScore W1576964650C552990157 @default.
- W1576964650 hasConceptScore W1576964650C78063203 @default.
- W1576964650 hasConceptScore W1576964650C86803240 @default.
- W1576964650 hasConceptScore W1576964650C89423630 @default.
- W1576964650 hasIssue "2" @default.
- W1576964650 hasLocation W15769646501 @default.
- W1576964650 hasOpenAccess W1576964650 @default.
- W1576964650 hasPrimaryLocation W15769646501 @default.
- W1576964650 hasRelatedWork W114802792 @default.
- W1576964650 hasRelatedWork W1520685780 @default.
- W1576964650 hasRelatedWork W168352937 @default.
- W1576964650 hasRelatedWork W1879038635 @default.
- W1576964650 hasRelatedWork W1974803925 @default.
- W1576964650 hasRelatedWork W2018973640 @default.
- W1576964650 hasRelatedWork W2052745879 @default.
- W1576964650 hasRelatedWork W2095463674 @default.
- W1576964650 hasRelatedWork W2098148503 @default.
- W1576964650 hasRelatedWork W2113743041 @default.
- W1576964650 hasRelatedWork W2121574136 @default.
- W1576964650 hasRelatedWork W2123449092 @default.
- W1576964650 hasRelatedWork W2142401436 @default.
- W1576964650 hasRelatedWork W2156437009 @default.
- W1576964650 hasRelatedWork W2363477247 @default.
- W1576964650 hasRelatedWork W2384319862 @default.
- W1576964650 hasRelatedWork W2389059028 @default.
- W1576964650 hasRelatedWork W270607214 @default.
- W1576964650 hasRelatedWork W350389720 @default.
- W1576964650 hasRelatedWork W157851036 @default.
- W1576964650 hasVolume "41" @default.
- W1576964650 isParatext "false" @default.
- W1576964650 isRetracted "false" @default.
- W1576964650 magId "1576964650" @default.
- W1576964650 workType "article" @default.