FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1577491827> ?p ?o ?g. }

• W1577491827 endingPage "10184" @default.
• W1577491827 startingPage "10180" @default.
• W1577491827 abstract "The purpose of this work was to study vasoactive intestinal peptide (VIP) receptors and the adenylate cyclase response to VIP upon enterocytic differentiation of the human colon adenocarcinoma cell line Caco-2 in culture. The VIP-stimulated enzyme activity is very low, e.g. 20% above basal activity in undifferentiated cells (day 5) and is enhanced markedly at confluency reaching a maximum, e.g. 270%, above basal activity in fully differentiated cells (day 30). VIP potency is also slightly enhanced, the EC50 of VIP ranging from 0.31 nM at day 5 to 0.07 nM at day 30. Modifications of the adenylate cyclase system are not responsible for the development of VIP response. Indeed, forskolin-stimulated adenylate cyclase activity is unchanged during differentiation supporting no alteration of the enzyme catalytic subunit. The same holds true for NaF and guanosine 5'-(beta, gamma-imido)trisphosphate, indicating a constant activity of the guanine nucleotide regulatory unit which mediates hormonal stimulation of adenylate cyclase (Ns). This is further supported by the similar extent of cholera toxin-catalyzed [32P]ADP-ribosylation of the Ns protein that is observed during differentiation. In sharp contrast, a dramatic increase of VIP receptor concentration is observed ranging from 32 fmol/mg of protein at day 5 to 414 fmol/mg of protein at day 30. This is confirmed by affinity cross-linking experiments showing an increased specific incorporation of 125I-VIP in a major 66,000-dalton component during differentiation. A slight increase in receptor affinity is also observed during differentiation with Kd ranging from 0.39 nM at day 5 to 0.08 nM at day 30. These data indicate that one population of VIP receptors accumulates during Caco-2 cell differentiation, representing the crucial event in the development of adenylate cyclase response to the peptide." @default.
• W1577491827 created "2016-06-24" @default.
• W1577491827 creator A5003217193 @default.
• W1577491827 creator A5012381902 @default.
• W1577491827 creator A5026490340 @default.
• W1577491827 creator A5043156688 @default.
• W1577491827 creator A5061688223 @default.
• W1577491827 creator A5074672318 @default.
• W1577491827 creator A5076634867 @default.
• W1577491827 date "1987-07-01" @default.
• W1577491827 modified "2023-10-17" @default.
• W1577491827 title "Development of vasoactive intestinal peptide-responsive adenylate cyclase during enterocytic differentiation of Caco-2 cells in culture. Evidence for an increased receptor level." @default.
• W1577491827 cites W111440207 @default.
• W1577491827 cites W12110462 @default.
• W1577491827 cites W1480933474 @default.
• W1577491827 cites W1491339323 @default.
• W1577491827 cites W1495187283 @default.
• W1577491827 cites W1511336559 @default.
• W1577491827 cites W1531015551 @default.
• W1577491827 cites W1536268728 @default.
• W1577491827 cites W1587225564 @default.
• W1577491827 cites W1625058931 @default.
• W1577491827 cites W1964014423 @default.
• W1577491827 cites W1964460699 @default.
• W1577491827 cites W1973972465 @default.
• W1577491827 cites W1977658178 @default.
• W1577491827 cites W1978752676 @default.
• W1577491827 cites W1983649348 @default.
• W1577491827 cites W1984080990 @default.
• W1577491827 cites W1995924566 @default.
• W1577491827 cites W1997627653 @default.
• W1577491827 cites W1999572341 @default.
• W1577491827 cites W2009291798 @default.
• W1577491827 cites W2012345358 @default.
• W1577491827 cites W2024296404 @default.
• W1577491827 cites W2027073741 @default.
• W1577491827 cites W2038772881 @default.
• W1577491827 cites W2042871417 @default.
• W1577491827 cites W2061051288 @default.
• W1577491827 cites W2073519885 @default.
• W1577491827 cites W2075676530 @default.
• W1577491827 cites W2088890372 @default.
• W1577491827 cites W2089264375 @default.
• W1577491827 cites W2100837269 @default.
• W1577491827 cites W2106448082 @default.
• W1577491827 cites W2118678170 @default.
• W1577491827 cites W2120640452 @default.
• W1577491827 cites W2121192663 @default.
• W1577491827 cites W2128982679 @default.
• W1577491827 cites W2404082640 @default.
• W1577491827 cites W4293247451 @default.
• W1577491827 doi "https://doi.org/10.1016/s0021-9258(18)61094-4" @default.
• W1577491827 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3038870" @default.
• W1577491827 hasPublicationYear "1987" @default.
• W1577491827 type Work @default.
• W1577491827 sameAs 1577491827 @default.
• W1577491827 citedByCount "61" @default.
• W1577491827 crossrefType "journal-article" @default.
• W1577491827 hasAuthorship W1577491827A5003217193 @default.
• W1577491827 hasAuthorship W1577491827A5012381902 @default.
• W1577491827 hasAuthorship W1577491827A5026490340 @default.
• W1577491827 hasAuthorship W1577491827A5043156688 @default.
• W1577491827 hasAuthorship W1577491827A5061688223 @default.
• W1577491827 hasAuthorship W1577491827A5074672318 @default.
• W1577491827 hasAuthorship W1577491827A5076634867 @default.
• W1577491827 hasBestOaLocation W15774918271 @default.
• W1577491827 hasConcept C118303440 @default.
• W1577491827 hasConcept C134018914 @default.
• W1577491827 hasConcept C1491633281 @default.
• W1577491827 hasConcept C170493617 @default.
• W1577491827 hasConcept C180526460 @default.
• W1577491827 hasConcept C185592680 @default.
• W1577491827 hasConcept C193176779 @default.
• W1577491827 hasConcept C2780069284 @default.
• W1577491827 hasConcept C55493867 @default.
• W1577491827 hasConcept C8330315 @default.
• W1577491827 hasConcept C86803240 @default.
• W1577491827 hasConcept C95444343 @default.
• W1577491827 hasConceptScore W1577491827C118303440 @default.
• W1577491827 hasConceptScore W1577491827C134018914 @default.
• W1577491827 hasConceptScore W1577491827C1491633281 @default.
• W1577491827 hasConceptScore W1577491827C170493617 @default.
• W1577491827 hasConceptScore W1577491827C180526460 @default.
• W1577491827 hasConceptScore W1577491827C185592680 @default.
• W1577491827 hasConceptScore W1577491827C193176779 @default.
• W1577491827 hasConceptScore W1577491827C2780069284 @default.
• W1577491827 hasConceptScore W1577491827C55493867 @default.
• W1577491827 hasConceptScore W1577491827C8330315 @default.
• W1577491827 hasConceptScore W1577491827C86803240 @default.
• W1577491827 hasConceptScore W1577491827C95444343 @default.
• W1577491827 hasIssue "21" @default.
• W1577491827 hasLocation W15774918271 @default.
• W1577491827 hasOpenAccess W1577491827 @default.
• W1577491827 hasPrimaryLocation W15774918271 @default.
• W1577491827 hasRelatedWork W1995462424 @default.
• W1577491827 hasRelatedWork W2005735134 @default.
• W1577491827 hasRelatedWork W2018953089 @default.
• W1577491827 hasRelatedWork W2079913068 @default.

• next