FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1577673150> ?p ?o ?g. }

• W1577673150 endingPage "15" @default.
• W1577673150 startingPage "9" @default.
• W1577673150 abstract "A new high-throughput GenomeLab Gene Expression Profiler (GeXP) analyser-based multiplex PCR assay was developed for the detection of eight reproductive and respiratory pathogens in swine. The reproductive and respiratory pathogens include North American porcine reproductive and respiratory syndrome virus (PRRSV-NA), classical swine fever virus (CSFV), porcine circovirus 2 (PCV-2), swine influenza virus (SIV) (including H1 and H3 subtypes), porcine parvovirus (PPV), pseudorabies virus (PRV) and Japanese encephalitis virus (JEV). Nine pairs of specific chimeric primers were designed and used to initiate PCRs, and one pair of universal primers was used for subsequent PCR cycles. The specificity of the GeXP assay was examined using positive controls for each virus. The sensitivity was evaluated using serial ten-fold dilutions of in vitro-transcribed RNA from all of the RNA viruses and plasmids from DNA viruses. The GeXP assay was further evaluated using 114 clinical specimens and was compared with real-time PCR/single RT-PCR methods. The specificity of the GeXP assay for each pathogen was examined using single cDNA/DNA template. Specific amplification peaks of the reproductive and respiratory pathogens were observed on the GeXP analyser. The minimum copies per reaction detected for each virus by the GeXP assay were as follows: 1000 copies/μl for PRV; 100 copies/μl for CSFV, JEV, PCV-2 and PPV; and 10 copies/μl for SIV-H1, SIV-H3 and PRRSV-NA. Analysis of 114 clinical samples using the GeXP assay demonstrated that the GeXP assay had comparable detection to real-time PCR/single RT-PCR. This study demonstrated that the GeXP assay is a new method with high sensitivity and specificity for the identification of these swine reproductive and respiratory pathogens. The GeXP assay may be adopted for molecular epidemiological surveys of these reproductive and respiratory pathogens in swine populations." @default.
• W1577673150 created "2016-06-24" @default.
• W1577673150 creator A5006124324 @default.
• W1577673150 creator A5017617784 @default.
• W1577673150 creator A5027365853 @default.
• W1577673150 creator A5045443752 @default.
• W1577673150 creator A5067756468 @default.
• W1577673150 creator A5076596429 @default.
• W1577673150 creator A5078760161 @default.
• W1577673150 creator A5084535169 @default.
• W1577673150 creator A5088768408 @default.
• W1577673150 date "2015-11-01" @default.
• W1577673150 modified "2023-09-27" @default.
• W1577673150 title "Simultaneous detection of eight swine reproductive and respiratory pathogens using a novel GeXP analyser-based multiplex PCR assay" @default.
• W1577673150 cites W1968017175 @default.
• W1577673150 cites W1969187577 @default.
• W1577673150 cites W1972729986 @default.
• W1577673150 cites W1978816241 @default.
• W1577673150 cites W1984119182 @default.
• W1577673150 cites W1984208004 @default.
• W1577673150 cites W1988578011 @default.
• W1577673150 cites W1989299578 @default.
• W1577673150 cites W1993677955 @default.
• W1577673150 cites W1998003003 @default.
• W1577673150 cites W2003849514 @default.
• W1577673150 cites W2008372272 @default.
• W1577673150 cites W2010499884 @default.
• W1577673150 cites W2013629471 @default.
• W1577673150 cites W2023542080 @default.
• W1577673150 cites W2024557473 @default.
• W1577673150 cites W2030485140 @default.
• W1577673150 cites W2036522696 @default.
• W1577673150 cites W2053776454 @default.
• W1577673150 cites W2125427170 @default.
• W1577673150 cites W2164770701 @default.
• W1577673150 cites W2172299258 @default.
• W1577673150 cites W2187704513 @default.
• W1577673150 cites W2195941368 @default.
• W1577673150 cites W4234856234 @default.
• W1577673150 doi "https://doi.org/10.1016/j.jviromet.2015.08.001" @default.
• W1577673150 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26259690" @default.
• W1577673150 hasPublicationYear "2015" @default.
• W1577673150 type Work @default.
• W1577673150 sameAs 1577673150 @default.
• W1577673150 citedByCount "18" @default.
• W1577673150 countsByYear W15776731502015 @default.
• W1577673150 countsByYear W15776731502016 @default.
• W1577673150 countsByYear W15776731502017 @default.
• W1577673150 countsByYear W15776731502018 @default.
• W1577673150 countsByYear W15776731502019 @default.
• W1577673150 countsByYear W15776731502021 @default.
• W1577673150 crossrefType "journal-article" @default.
• W1577673150 hasAuthorship W1577673150A5006124324 @default.
• W1577673150 hasAuthorship W1577673150A5017617784 @default.
• W1577673150 hasAuthorship W1577673150A5027365853 @default.
• W1577673150 hasAuthorship W1577673150A5045443752 @default.
• W1577673150 hasAuthorship W1577673150A5067756468 @default.
• W1577673150 hasAuthorship W1577673150A5076596429 @default.
• W1577673150 hasAuthorship W1577673150A5078760161 @default.
• W1577673150 hasAuthorship W1577673150A5084535169 @default.
• W1577673150 hasAuthorship W1577673150A5088768408 @default.
• W1577673150 hasConcept C104317684 @default.
• W1577673150 hasConcept C159047783 @default.
• W1577673150 hasConcept C2522874641 @default.
• W1577673150 hasConcept C2778951572 @default.
• W1577673150 hasConcept C2779005923 @default.
• W1577673150 hasConcept C2779141303 @default.
• W1577673150 hasConcept C2779819720 @default.
• W1577673150 hasConcept C2781045504 @default.
• W1577673150 hasConcept C2781092067 @default.
• W1577673150 hasConcept C2781188995 @default.
• W1577673150 hasConcept C48023723 @default.
• W1577673150 hasConcept C49105822 @default.
• W1577673150 hasConcept C54355233 @default.
• W1577673150 hasConcept C83455156 @default.
• W1577673150 hasConcept C86803240 @default.
• W1577673150 hasConceptScore W1577673150C104317684 @default.
• W1577673150 hasConceptScore W1577673150C159047783 @default.
• W1577673150 hasConceptScore W1577673150C2522874641 @default.
• W1577673150 hasConceptScore W1577673150C2778951572 @default.
• W1577673150 hasConceptScore W1577673150C2779005923 @default.
• W1577673150 hasConceptScore W1577673150C2779141303 @default.
• W1577673150 hasConceptScore W1577673150C2779819720 @default.
• W1577673150 hasConceptScore W1577673150C2781045504 @default.
• W1577673150 hasConceptScore W1577673150C2781092067 @default.
• W1577673150 hasConceptScore W1577673150C2781188995 @default.
• W1577673150 hasConceptScore W1577673150C48023723 @default.
• W1577673150 hasConceptScore W1577673150C49105822 @default.
• W1577673150 hasConceptScore W1577673150C54355233 @default.
• W1577673150 hasConceptScore W1577673150C83455156 @default.
• W1577673150 hasConceptScore W1577673150C86803240 @default.
• W1577673150 hasLocation W15776731501 @default.
• W1577673150 hasLocation W15776731502 @default.
• W1577673150 hasOpenAccess W1577673150 @default.
• W1577673150 hasPrimaryLocation W15776731501 @default.
• W1577673150 hasRelatedWork W2008968043 @default.
• W1577673150 hasRelatedWork W2036522696 @default.
• W1577673150 hasRelatedWork W2355487793 @default.

• next