Matches in SemOpenAlex for { <https://semopenalex.org/work/W1578114531> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1578114531 endingPage "10872" @default.
- W1578114531 startingPage "10867" @default.
- W1578114531 abstract "In Saccharomyces cerevisiae, trehalase activity in crude extracts obtained from wild type cells was activated about 3-fold by preincubation with cAMP and ATP. The inactive trehalase fractionated by DEAE-Sephacel chromatography was activated by the addition of the cAMP-dependent protein kinase fraction from wild type cells in the presence of cAMP and ATP. Using the crude extract obtained from bcy1 mutant cells which were deficient in the regulatory subunit of cAMP-dependent protein kinase, the stimulation of trehalase activity was observed in the absence of cAMP. The cAMP-dependent protein kinase of CYR3 mutant cells which had a high Ka value for cAMP in the phosphorylation reaction required a high cAMP concentration for activation of trehalase. Increased activation of partially purified inactive trehalase (Mr = 320,000) was observed to correlate with increased phosphorylation of a protein (Mr = 80,000) identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The assay results using various mutants altered in cAMP metabolism indicated that the activation and phosphorylation of inactive trehalase fractions depended on the cAMP concentration accumulated in mutant cells. Inactivation and dephosphorylation of active trehalase fractions were observed by treatment with alkaline phosphatase or crude cell extracts. The results indicated that the conversion of inactive form of trehalase to the active form is regulated by cAMP through cAMP-dependent protein kinase." @default.
- W1578114531 created "2016-06-24" @default.
- W1578114531 creator A5005059279 @default.
- W1578114531 creator A5027022285 @default.
- W1578114531 creator A5038015812 @default.
- W1578114531 creator A5048428374 @default.
- W1578114531 date "1983-09-01" @default.
- W1578114531 modified "2023-10-03" @default.
- W1578114531 title "Genetic and biochemical evidence that trehalase is a substrate of cAMP-dependent protein kinase in yeast." @default.
- W1578114531 cites W109722222 @default.
- W1578114531 cites W1522666427 @default.
- W1578114531 cites W1525979122 @default.
- W1578114531 cites W1526252582 @default.
- W1578114531 cites W1527373967 @default.
- W1578114531 cites W1537442027 @default.
- W1578114531 cites W1753314824 @default.
- W1578114531 cites W1775749144 @default.
- W1578114531 cites W1869849339 @default.
- W1578114531 cites W1969241814 @default.
- W1578114531 cites W1971365679 @default.
- W1578114531 cites W2016062383 @default.
- W1578114531 cites W2022707104 @default.
- W1578114531 cites W2022752383 @default.
- W1578114531 cites W2029446144 @default.
- W1578114531 cites W2046511121 @default.
- W1578114531 cites W2072189230 @default.
- W1578114531 cites W2081866602 @default.
- W1578114531 cites W2084108403 @default.
- W1578114531 cites W2120331014 @default.
- W1578114531 cites W2125992715 @default.
- W1578114531 cites W4252973684 @default.
- W1578114531 doi "https://doi.org/10.1016/s0021-9258(17)44356-0" @default.
- W1578114531 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6309818" @default.
- W1578114531 hasPublicationYear "1983" @default.
- W1578114531 type Work @default.
- W1578114531 sameAs 1578114531 @default.
- W1578114531 citedByCount "144" @default.
- W1578114531 countsByYear W15781145312012 @default.
- W1578114531 countsByYear W15781145312013 @default.
- W1578114531 countsByYear W15781145312014 @default.
- W1578114531 countsByYear W15781145312015 @default.
- W1578114531 countsByYear W15781145312016 @default.
- W1578114531 countsByYear W15781145312017 @default.
- W1578114531 countsByYear W15781145312018 @default.
- W1578114531 countsByYear W15781145312019 @default.
- W1578114531 crossrefType "journal-article" @default.
- W1578114531 hasAuthorship W1578114531A5005059279 @default.
- W1578114531 hasAuthorship W1578114531A5027022285 @default.
- W1578114531 hasAuthorship W1578114531A5038015812 @default.
- W1578114531 hasAuthorship W1578114531A5048428374 @default.
- W1578114531 hasBestOaLocation W15781145311 @default.
- W1578114531 hasConcept C104292427 @default.
- W1578114531 hasConcept C104317684 @default.
- W1578114531 hasConcept C11960822 @default.
- W1578114531 hasConcept C153911025 @default.
- W1578114531 hasConcept C178666793 @default.
- W1578114531 hasConcept C181199279 @default.
- W1578114531 hasConcept C184235292 @default.
- W1578114531 hasConcept C185592680 @default.
- W1578114531 hasConcept C2777576037 @default.
- W1578114531 hasConcept C2779222958 @default.
- W1578114531 hasConcept C2779315325 @default.
- W1578114531 hasConcept C55493867 @default.
- W1578114531 hasConcept C86803240 @default.
- W1578114531 hasConcept C97029542 @default.
- W1578114531 hasConceptScore W1578114531C104292427 @default.
- W1578114531 hasConceptScore W1578114531C104317684 @default.
- W1578114531 hasConceptScore W1578114531C11960822 @default.
- W1578114531 hasConceptScore W1578114531C153911025 @default.
- W1578114531 hasConceptScore W1578114531C178666793 @default.
- W1578114531 hasConceptScore W1578114531C181199279 @default.
- W1578114531 hasConceptScore W1578114531C184235292 @default.
- W1578114531 hasConceptScore W1578114531C185592680 @default.
- W1578114531 hasConceptScore W1578114531C2777576037 @default.
- W1578114531 hasConceptScore W1578114531C2779222958 @default.
- W1578114531 hasConceptScore W1578114531C2779315325 @default.
- W1578114531 hasConceptScore W1578114531C55493867 @default.
- W1578114531 hasConceptScore W1578114531C86803240 @default.
- W1578114531 hasConceptScore W1578114531C97029542 @default.
- W1578114531 hasIssue "18" @default.
- W1578114531 hasLocation W15781145311 @default.
- W1578114531 hasOpenAccess W1578114531 @default.
- W1578114531 hasPrimaryLocation W15781145311 @default.
- W1578114531 hasRelatedWork W2009810460 @default.
- W1578114531 hasRelatedWork W2020828464 @default.
- W1578114531 hasRelatedWork W2025917340 @default.
- W1578114531 hasRelatedWork W2028243295 @default.
- W1578114531 hasRelatedWork W2032533427 @default.
- W1578114531 hasRelatedWork W2062385823 @default.
- W1578114531 hasRelatedWork W2087346710 @default.
- W1578114531 hasRelatedWork W2113444059 @default.
- W1578114531 hasRelatedWork W2465694941 @default.
- W1578114531 hasRelatedWork W2184344602 @default.
- W1578114531 hasVolume "258" @default.
- W1578114531 isParatext "false" @default.
- W1578114531 isRetracted "false" @default.
- W1578114531 magId "1578114531" @default.
- W1578114531 workType "article" @default.