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- W1578350484 abstract "Abstract The majority of particles of lettuce necrotic yellows virus stained in phosphotungstic acid (PTA) at high or low pH appear rounded in outline when examined in the electron microscope. However, when stained between pH 6 and 7.5, most particles are bacilliform or bullet-shaped. It is suggested that these differences in particle shape are due to imbibition and osmotic changes brought about by changes in pH. The length of the bacilliform particles was also found to be affected by the pH of PTA used in staining. Longer particles were observed in virus preparations stained at pH's below 4.5, and some evidence is presented that particles fragment when stained in PTA at higher pH. In PTA at pH below 5, virus particles stained densely whereas at higher pH the stain merely outlined the particles. Osmium fixation prior to staining tended to reduce the amount of stain taken up by virus particles. These observations may be explained in terms of changes in permeability of the viral envelope with changes of pH." @default.
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- W1578350484 date "1969-03-01" @default.
- W1578350484 modified "2023-09-27" @default.
- W1578350484 title "Structure of lettuce necrotic yellows virus" @default.
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- W1578350484 doi "https://doi.org/10.1016/0042-6822(69)90227-x" @default.
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