FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1578457202> ?p ?o ?g. }

• W1578457202 endingPage "931" @default.
• W1578457202 startingPage "925" @default.
• W1578457202 abstract "We have recently demonstrated that metalloporphyrins are potent inhibitors of both human immunodeficiency virus type 1 (HIV-1) and human immunodeficiency virus type 2 (HIV-2) reverse transcriptases (RTs) [Argyris, E.G., Vanderkooi, J.M., Venkateswaran, P.S., Kay, B.K., and Paterson, Y. (1999) J. Biol. Chem. 274, 1549-1556]. In addition, by screening a phage peptide library we discovered that a peptide with sequence similarity to residues 398-407 from the connection subdomain of HIV RTs binds heme. These findings suggested that this highly conserved region may be the binding site for metalloporphyrins and a novel site for inhibition of enzymatic activity. Our most recent data presented here confirm this suggestion. Screening of HIV-1 RT 398-407 peptide analogs by fluorescence assays demonstrates that Trp residues at positions 401 and 402 are important for heme binding. Furthermore, site-directed mutagenesis of these residues verified these findings and indicated that heme inhibits HIV-1 RT by binding on the connection subdomain of the p66 subunit of the enzyme but not on the p51 subunit. This was also confirmed by analyzing the binding affinities of heme for mutant HIV-1 RT heterodimers, using intrinsic fluorescence assays. The clear identification of the connection domain as a novel inhibition site is crucial in understanding the mechanism of heme binding and enzymatic inhibition and will facilitate the generation of novel porphyrin-based inhibitors of RT." @default.
• W1578457202 created "2016-06-24" @default.
• W1578457202 creator A5001173869 @default.
• W1578457202 creator A5043702675 @default.
• W1578457202 creator A5091812025 @default.
• W1578457202 date "2001-02-01" @default.
• W1578457202 modified "2023-10-18" @default.
• W1578457202 title "Mutagenesis of key residues identifies the connection subdomain of HIV-1 reverse transcriptase as the site of inhibition by heme" @default.
• W1578457202 cites W1502628406 @default.
• W1578457202 cites W1546361071 @default.
• W1578457202 cites W1603726506 @default.
• W1578457202 cites W1969885133 @default.
• W1578457202 cites W1976580962 @default.
• W1578457202 cites W1981765089 @default.
• W1578457202 cites W1984298556 @default.
• W1578457202 cites W1984814223 @default.
• W1578457202 cites W1985783475 @default.
• W1578457202 cites W1987596112 @default.
• W1578457202 cites W2008954911 @default.
• W1578457202 cites W2028143521 @default.
• W1578457202 cites W2032026161 @default.
• W1578457202 cites W2034209707 @default.
• W1578457202 cites W2046134251 @default.
• W1578457202 cites W2055542167 @default.
• W1578457202 cites W2060913542 @default.
• W1578457202 cites W2063176659 @default.
• W1578457202 cites W2069815958 @default.
• W1578457202 cites W2074495023 @default.
• W1578457202 cites W2074864312 @default.
• W1578457202 cites W2079604571 @default.
• W1578457202 cites W2084702672 @default.
• W1578457202 cites W2085459671 @default.
• W1578457202 cites W2087411521 @default.
• W1578457202 cites W2089024107 @default.
• W1578457202 cites W2092786021 @default.
• W1578457202 cites W2102098015 @default.
• W1578457202 cites W2102973576 @default.
• W1578457202 cites W2105634098 @default.
• W1578457202 cites W2124217674 @default.
• W1578457202 cites W2156591083 @default.
• W1578457202 cites W2405525952 @default.
• W1578457202 cites W2417132973 @default.
• W1578457202 cites W3024210805 @default.
• W1578457202 doi "https://doi.org/10.1046/j.1432-1327.2001.01944.x" @default.
• W1578457202 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11179958" @default.
• W1578457202 hasPublicationYear "2001" @default.
• W1578457202 type Work @default.
• W1578457202 sameAs 1578457202 @default.
• W1578457202 citedByCount "12" @default.
• W1578457202 countsByYear W15784572022012 @default.
• W1578457202 countsByYear W15784572022015 @default.
• W1578457202 countsByYear W15784572022019 @default.
• W1578457202 countsByYear W15784572022021 @default.
• W1578457202 countsByYear W15784572022022 @default.
• W1578457202 crossrefType "journal-article" @default.
• W1578457202 hasAuthorship W1578457202A5001173869 @default.
• W1578457202 hasAuthorship W1578457202A5043702675 @default.
• W1578457202 hasAuthorship W1578457202A5091812025 @default.
• W1578457202 hasBestOaLocation W15784572021 @default.
• W1578457202 hasConcept C103408237 @default.
• W1578457202 hasConcept C104292427 @default.
• W1578457202 hasConcept C104317684 @default.
• W1578457202 hasConcept C107824862 @default.
• W1578457202 hasConcept C143065580 @default.
• W1578457202 hasConcept C156719811 @default.
• W1578457202 hasConcept C16318435 @default.
• W1578457202 hasConcept C181199279 @default.
• W1578457202 hasConcept C185592680 @default.
• W1578457202 hasConcept C2776217839 @default.
• W1578457202 hasConcept C2779281246 @default.
• W1578457202 hasConcept C41183919 @default.
• W1578457202 hasConcept C55493867 @default.
• W1578457202 hasConcept C67705224 @default.
• W1578457202 hasConcept C86803240 @default.
• W1578457202 hasConceptScore W1578457202C103408237 @default.
• W1578457202 hasConceptScore W1578457202C104292427 @default.
• W1578457202 hasConceptScore W1578457202C104317684 @default.
• W1578457202 hasConceptScore W1578457202C107824862 @default.
• W1578457202 hasConceptScore W1578457202C143065580 @default.
• W1578457202 hasConceptScore W1578457202C156719811 @default.
• W1578457202 hasConceptScore W1578457202C16318435 @default.
• W1578457202 hasConceptScore W1578457202C181199279 @default.
• W1578457202 hasConceptScore W1578457202C185592680 @default.
• W1578457202 hasConceptScore W1578457202C2776217839 @default.
• W1578457202 hasConceptScore W1578457202C2779281246 @default.
• W1578457202 hasConceptScore W1578457202C41183919 @default.
• W1578457202 hasConceptScore W1578457202C55493867 @default.
• W1578457202 hasConceptScore W1578457202C67705224 @default.
• W1578457202 hasConceptScore W1578457202C86803240 @default.
• W1578457202 hasIssue "4" @default.
• W1578457202 hasLocation W15784572021 @default.
• W1578457202 hasLocation W15784572022 @default.
• W1578457202 hasOpenAccess W1578457202 @default.
• W1578457202 hasPrimaryLocation W15784572021 @default.
• W1578457202 hasRelatedWork W1578457202 @default.
• W1578457202 hasRelatedWork W1974993927 @default.
• W1578457202 hasRelatedWork W1995916976 @default.
• W1578457202 hasRelatedWork W2017218857 @default.

• next