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- W1578463576 abstract "Drug metabolism and phomacokinetics of biologics has been an increasingly interesting topic. LC-MS techniques can potentially provide more accurate understanding of the biotransformation of therapeutic proteins and complimentary information to ELISA methodologies. Although, using LC-MS/MS as the technology for small molecule DMPK has been established in the industry, using LC-MS as a qualititative and quantitative tool for large molecules DMPK have not been widely accepted. In this abstract, a model protein, Lysozyme and model peptide MUC5AC-13 were spiked into human serum. An analog of MUC5AC-13 was spiked in as an internal calibration standard. A high resolution Q-TOF mass spectrometry was used to acquire data. For the peptide, LOQ of 50pg on 2.1mm column and 4pg on 75um HPLC-Chip were achieved. Average RSD was 4.2% with three orders of magnitude linearity. At the same time, MS/MS provided qualitative information for the validation the peptide sequence. For model protein lysozyme, linearity was achieved from 1 – 1000ug/mL in TOF scan mode. These results have shown excellent potential of Q-TOF as a Qual/Quant technique for biologic samples." @default.
- W1578463576 created "2016-06-24" @default.
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- W1578463576 date "2012-01-01" @default.
- W1578463576 modified "2023-09-23" @default.
- W1578463576 title "Quantification of a Model Protein Lysozyme and Peptide MUC5AC-13 in Serum Matrix Using LC-QTOF." @default.
- W1578463576 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3630653" @default.
- W1578463576 hasPublicationYear "2012" @default.
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