Matches in SemOpenAlex for { <https://semopenalex.org/work/W1578973180> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W1578973180 endingPage "3764" @default.
- W1578973180 startingPage "3755" @default.
- W1578973180 abstract "Abstract Class I MHC Ag presentation and cell surface expression largely depend on peptide transport into the ER/cis-Golgi by TAP, the transporter associated with Ag processing. Despite this dependency, class I MHC molecules are expressed at low levels on the surface of TAP-deficient T2 cells. We studied the peptide dependency of HLA-B7 expression in transfected T2 cells. HLA-B7 expression was affected by mutations at 19 out of 23 peptide-binding groove residues, but not by nine mutations outside of the peptide-binding groove. T2 cell surface HLA-A2, -B7, and -B51 had similar stabilities, and approximately half of these class I molecules had a long t1/2 consistent with tight peptide binding. Using metabolically labeled T2 cells, HLA-A2-bound peptide eluted as five prominent peaks, but HLA-B7-bound peptide was not detected. In contrast, HLA-B7-eluted peptides were detected spectrophotometrically. These data suggest that HLA-A2 and HLA-B7 molecules utilize distinct TAP-independent peptide supply mechanisms to different degrees. Equivalent amounts of HLA-B7 from TAP- and TAP+ cells yielded similar amounts of peptide, which had the characteristic HLA-B7 peptide-binding motif. The dependency of HLA-B7 cell surface expression on peptide-binding groove residues, the stability of cell surface class I molecules, and the ability to detect HLA-B7-bound peptide indicate that the low level expression on T2 cells is largely peptide dependent. TAP-independent peptide Ag presentation may allow immune recognition of intracellular pathogens that interfere with TAP-dependent peptide transport." @default.
- W1578973180 created "2016-06-24" @default.
- W1578973180 creator A5027246219 @default.
- W1578973180 creator A5079014789 @default.
- W1578973180 date "1996-05-15" @default.
- W1578973180 modified "2023-10-01" @default.
- W1578973180 title "Peptide-dependent expression of HLA-B7 on antigen processing-deficient T2 cells." @default.
- W1578973180 doi "https://doi.org/10.4049/jimmunol.156.10.3755" @default.
- W1578973180 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8621911" @default.
- W1578973180 hasPublicationYear "1996" @default.
- W1578973180 type Work @default.
- W1578973180 sameAs 1578973180 @default.
- W1578973180 citedByCount "30" @default.
- W1578973180 countsByYear W15789731802013 @default.
- W1578973180 countsByYear W15789731802019 @default.
- W1578973180 countsByYear W15789731802020 @default.
- W1578973180 countsByYear W15789731802023 @default.
- W1578973180 crossrefType "journal-article" @default.
- W1578973180 hasAuthorship W1578973180A5027246219 @default.
- W1578973180 hasAuthorship W1578973180A5079014789 @default.
- W1578973180 hasConcept C104317684 @default.
- W1578973180 hasConcept C108249834 @default.
- W1578973180 hasConcept C147483822 @default.
- W1578973180 hasConcept C153911025 @default.
- W1578973180 hasConcept C154317977 @default.
- W1578973180 hasConcept C170627219 @default.
- W1578973180 hasConcept C185592680 @default.
- W1578973180 hasConcept C188280979 @default.
- W1578973180 hasConcept C202751555 @default.
- W1578973180 hasConcept C203014093 @default.
- W1578973180 hasConcept C207936829 @default.
- W1578973180 hasConcept C22845430 @default.
- W1578973180 hasConcept C2779281246 @default.
- W1578973180 hasConcept C55493867 @default.
- W1578973180 hasConcept C83464605 @default.
- W1578973180 hasConcept C86803240 @default.
- W1578973180 hasConceptScore W1578973180C104317684 @default.
- W1578973180 hasConceptScore W1578973180C108249834 @default.
- W1578973180 hasConceptScore W1578973180C147483822 @default.
- W1578973180 hasConceptScore W1578973180C153911025 @default.
- W1578973180 hasConceptScore W1578973180C154317977 @default.
- W1578973180 hasConceptScore W1578973180C170627219 @default.
- W1578973180 hasConceptScore W1578973180C185592680 @default.
- W1578973180 hasConceptScore W1578973180C188280979 @default.
- W1578973180 hasConceptScore W1578973180C202751555 @default.
- W1578973180 hasConceptScore W1578973180C203014093 @default.
- W1578973180 hasConceptScore W1578973180C207936829 @default.
- W1578973180 hasConceptScore W1578973180C22845430 @default.
- W1578973180 hasConceptScore W1578973180C2779281246 @default.
- W1578973180 hasConceptScore W1578973180C55493867 @default.
- W1578973180 hasConceptScore W1578973180C83464605 @default.
- W1578973180 hasConceptScore W1578973180C86803240 @default.
- W1578973180 hasIssue "10" @default.
- W1578973180 hasLocation W15789731801 @default.
- W1578973180 hasLocation W15789731802 @default.
- W1578973180 hasOpenAccess W1578973180 @default.
- W1578973180 hasPrimaryLocation W15789731801 @default.
- W1578973180 hasRelatedWork W1592776416 @default.
- W1578973180 hasRelatedWork W1991142812 @default.
- W1578973180 hasRelatedWork W1992901347 @default.
- W1578973180 hasRelatedWork W2023967363 @default.
- W1578973180 hasRelatedWork W2039266996 @default.
- W1578973180 hasRelatedWork W2044070030 @default.
- W1578973180 hasRelatedWork W2058264657 @default.
- W1578973180 hasRelatedWork W2069829480 @default.
- W1578973180 hasRelatedWork W2409344746 @default.
- W1578973180 hasRelatedWork W2462657557 @default.
- W1578973180 hasVolume "156" @default.
- W1578973180 isParatext "false" @default.
- W1578973180 isRetracted "false" @default.
- W1578973180 magId "1578973180" @default.
- W1578973180 workType "article" @default.