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- W1579182009 abstract "The objective of our study is to explore the potential of poly (amidoamine) (PAMAM) dendrimers as a bonded stationary phase in open tubular capillary electrochromatography (CEC). 1G, 2G and 3G poly(amidoamine) (PAMAM) dendrimers were bonded to the interior wall of silica capillaries through a 3-aminopropyhriethoxysilane linker step by step. The obtained columns were characterized by CEC process. The results show that the electroosmotic flow (EOF) gradually reduced with increasing PAMAM generation. The performances of the column were evaluated by the separation of alanine and praline. The results indicate that the resolution of alanine (Ala) and proline (Pro) was gradually improved with increasing the amount of PAMAM generation. By open-tubular capillary electrochromatography column modified with the 3rd generation PAMAM, the baseline separation of the alanine and proline can be obtained. Five underivatized amino acids, arginine(Arg), alanine(Ala), proline(Pro), histidine(His) and methionine(Met), were successfully separated by OCEC modified with 3G PAMAM and by using UV detection at 200 nm. The reproducibility and stability of the column were satisfactory." @default.
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- W1579182009 date "2006-01-10" @default.
- W1579182009 modified "2023-10-16" @default.
- W1579182009 title "以枝形大分子聚酰胺-胺(PAMAM)为键合固定相的开管毛细管电色谱柱的制备及评价" @default.
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