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• W1579968991 abstract "Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this ε-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The ε-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The ε-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage." @default.
• W1579968991 created "2016-06-24" @default.
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• W1579968991 date "2013-08-30" @default.
• W1579968991 modified "2023-10-16" @default.
• W1579968991 title "Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice" @default.
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• W1579968991 doi "https://doi.org/10.1002/dvg.22420" @default.
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