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- W1580492641 abstract "This chapter provides a few general guidelines on sampling, subsampling, and other relevant field experimental design criteria on marine microorganisms. Many distinct marine ecosystems and their microbial assemblages are identified and studied, ranging from ice-swept polar seas to deep-sea hydrothermal vents. Sampling is one of the most important, but often overlooked, aspects of oceanography. The successful isolation of deep-sea bacterial isolates that are obligately barophilic has emphasized that pressure is an important determinant of microbiological zonation in the sea. Many techniques currently employed in microbiological oceanography require incubation of a seawater sample for various periods of time. The underlying assumption of these methods is that the subsequent incubation conditions do not alter the in situ rates of metabolism or biosynthesis. An equally important concern, especially for post-collection incubation measurements, is attention to a clean sampling technique. Metal samplers, toxic closure components or other potentially detrimental materials should be avoided. In order to ensure that the rates measured during the post-collection incubation procedure are representative of those occurring in nature, several precautions must be taken." @default.
- W1580492641 created "2016-06-24" @default.
- W1580492641 creator A5047717233 @default.
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- W1580492641 date "2001-01-01" @default.
- W1580492641 modified "2023-10-01" @default.
- W1580492641 title "Microbial ecology at sea: Sampling, subsampling and incubation considerations" @default.
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- W1580492641 doi "https://doi.org/10.1016/s0580-9517(01)30037-5" @default.
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