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- W1580494291 abstract "Current practice of indiscriminate and illegal felling of 'karas' trees (Aquilaria malaccensis) to extract 'gaharu' in the wild have caused the population to dwindle drastically. 'Gaharu' formed as a result of natural defense mechanism in trees, which unfortunately happens over a long period of time. To better understand natural processes of gaharu-making, we sought to study the roles of defense genes in 'karas' by cloning a gene that synthesizes secondary compounds. The phenylalanine ammonia-lyase (PAL) gene encodes an enzyme in the metabolism of phenylpropanoid compounds, which are produced in response to stress, such as damage by wounding and pathogen attack. We report a cloned PAL gene fragment from A. malaccensis using the polymerase chain reaction (PCR) technique. The fragment was amplified from genomic DNA of a wounded 2-year old tree and cloned into the pGEM-T Easy Vector. The cloned fragment had a length of 610bp and an uninterrupted open reading frame of 201 amino acids. When searched against the Genebank database using BLAST tools, the cloned fragment shared 92% sequence similarity at amino acid level to known PAL proteins. This work will serve as a foundation in identifying important genes for triggering 'gaharu' synthesis." @default.
- W1580494291 created "2016-06-24" @default.
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- W1580494291 date "2009-01-01" @default.
- W1580494291 modified "2023-09-23" @default.
- W1580494291 title "Cloning of Phenylalanine ammonia-lyase (PAL) gene fragment from Aquilaria malaccensis Lam. (karas)." @default.
- W1580494291 hasPublicationYear "2009" @default.
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