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• W1580497874 abstract "Chagas disease is a parasitic illness endemic in Latin America caused by the trypanosomatid parasite Trypanosoma cruzi that spreads around the world due to people migration. Nowadays, Benznidazole and Nifurtimox (banned in Brazil), are used for the treatment of this disease but causes severe side effects to patients. Recently, three new molecules have reached clinical trials phase in the development of drugs against Chagas disease but it is still necessary to develop new drugs. In this studies, the enzyme Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) was used as a target for the search of new antitrypanosomatid molecules. It belongs to the glycolytic pathway, the major one for parasite’s energy generation. With the aim searching a new molecule that inhibits this enzyme, the fragment-based approach guided the search of molecules with high ligand efficiency. A focused compound library was assembled from a database of 500,000 molecules using molecular and solubility filters and the Rule of Three. The integrated use of ligand (chemical and electrostatic similarity) and target (molecular docking) based drug design was carried out to rank the molecular fragments by a consensual score. Through visual inspection of the top 500 molecules five diverse fragments were selected for the in vitro enzymatic assays using fluorescence spectroscopy. One of these molecules shows a Ki equals to (425 ± 53) μM and ligand efficiency equals to 0,33, a promising value for the fragment-based approach. Additionally, Molecular Dynamics simulations (MD) were carried out with these fragments and the predicted energy of interaction for fragment-enzyme complex was able to rank the molecules as using the experimental results. Furthermore, the MD was useful to predict the mode of interaction of the fragments in the active site of enzyme and to reveal a new cavity close to the substrate binding site. A second generation of compounds was selected based on the structure of the active fragment to construct theoretical structure-activity relationship (SAR) using MD. SAR indicates that the presence of a nitrogen with hydrogen-bond donor property is important to the interaction, making hydrogen-bonding with the amino acid residue Asp210. In addition, MD shows the influence of different substituent position in the central ring in the energy of the interaction, with a 2,3 substitution at five-atom ring the most favorable. This study identifies the first molecular fragment with high ligand efficiency for the enzyme TcGAPDH, with the combined use of ligand and target-based tools and fluorescence spectroscopy, for selection and identification of active compounds against the enzyme. MD was able to reproduce experimental results and generate theoretical information of SAR to the active molecular fragment" @default.
• W1580497874 created "2016-06-24" @default.
• W1580497874 creator A5035333015 @default.
• W1580497874 date "2015-11-17" @default.
• W1580497874 modified "2023-09-23" @default.
• W1580497874 title "Planejamento de inibidores baseado em fragmentos moleculares para a enzima gliceraldeído-3-fosfato desidrogenase de Trypanosoma cruzi" @default.
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• W1580497874 doi "https://doi.org/10.11606/d.75.2012.tde-24072012-142746" @default.
• W1580497874 hasPublicationYear "2015" @default.
• W1580497874 type Work @default.
• W1580497874 sameAs 1580497874 @default.

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