FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1580614654> ?p ?o ?g. }

• W1580614654 endingPage "66" @default.
• W1580614654 startingPage "66" @default.
• W1580614654 abstract "Helper-dependent adenoviral vectors (HDAd) are attractive vectors for liver-directed gene therapy because they can mediate sustained, high level transgene expression with negligible long-term toxicity. However, high vector doses are required to achieve efficient hepatic transduction by peripheral intravenous injection due to a nonlinear dose response. Unfortunately, such high systemic doses result in wide spread vector dissemination and dose-dependent activation of the acute inflammatory response which can result in severe and lethal acute toxicity. We hypothesize that this problem can be surmounted by delivering the vector exclusively to the liver thereby permitting high efficiency hepatic transduction with low vector doses and with minimal systemic vector dissemination. To test this hypothesis, we injected HDAd directly into the surgically isolated liver via the portal vein in nonhuman primates. Total hepatic isolation was achieved by occluding hepatic blood inflow from the portal vein and hepatic artery and by occluding hepatic blood outflow at the vena cava. Prior to total hepatic isolation, saline was infused into the portal vein to flush blood out of the liver. The vector was then injected directly into the isolated liver via the portal vein and allowed to dwell for 30 min to prolong exposure to hepatocytes. Following the 30 min dwell time, unabsorbed vector was removed from the liver to minimize systemic dissemination. This was accomplished by infusing saline into the portal vein and collecting the unabsorbed vector from a vena cava catheter. We investigated the safety, feasibility and efficacy of this approach and our results revealed that the surgical procedure was well tolerated in nonhuman primates and that significantly higher hepatic transduction efficiencies can be achieved with relatively low vector doses as compared to those obtained by peripheral intravenous injection. This approach may increase the safety and efficacy of HDAd-mediated, liver-directed gene therapy by minimizing the dose required to achieve efficient hepatic transduction and by minimizing systemic vector dissemination." @default.
• W1580614654 created "2016-06-24" @default.
• W1580614654 creator A5002241022 @default.
• W1580614654 creator A5014863465 @default.
• W1580614654 creator A5019745578 @default.
• W1580614654 creator A5027157934 @default.
• W1580614654 creator A5035274699 @default.
• W1580614654 creator A5066761803 @default.
• W1580614654 creator A5071239607 @default.
• W1580614654 creator A5074963804 @default.
• W1580614654 creator A5078899373 @default.
• W1580614654 creator A5079182398 @default.
• W1580614654 date "2004-05-01" @default.
• W1580614654 modified "2023-09-27" @default.
• W1580614654 title "High efficiency HDAd-mediated hepatic transduction can be achieved by delivery into the surgically isolated liver of nonhuman primates" @default.
• W1580614654 doi "https://doi.org/10.1016/j.ymthe.2004.06.134" @default.
• W1580614654 hasPublicationYear "2004" @default.
• W1580614654 type Work @default.
• W1580614654 sameAs 1580614654 @default.
• W1580614654 citedByCount "0" @default.
• W1580614654 crossrefType "journal-article" @default.
• W1580614654 hasAuthorship W1580614654A5002241022 @default.
• W1580614654 hasAuthorship W1580614654A5014863465 @default.
• W1580614654 hasAuthorship W1580614654A5019745578 @default.
• W1580614654 hasAuthorship W1580614654A5027157934 @default.
• W1580614654 hasAuthorship W1580614654A5035274699 @default.
• W1580614654 hasAuthorship W1580614654A5066761803 @default.
• W1580614654 hasAuthorship W1580614654A5071239607 @default.
• W1580614654 hasAuthorship W1580614654A5074963804 @default.
• W1580614654 hasAuthorship W1580614654A5078899373 @default.
• W1580614654 hasAuthorship W1580614654A5079182398 @default.
• W1580614654 hasBestOaLocation W15806146541 @default.
• W1580614654 hasConcept C15152581 @default.
• W1580614654 hasConcept C169760540 @default.
• W1580614654 hasConcept C55493867 @default.
• W1580614654 hasConcept C86803240 @default.
• W1580614654 hasConcept C95444343 @default.
• W1580614654 hasConceptScore W1580614654C15152581 @default.
• W1580614654 hasConceptScore W1580614654C169760540 @default.
• W1580614654 hasConceptScore W1580614654C55493867 @default.
• W1580614654 hasConceptScore W1580614654C86803240 @default.
• W1580614654 hasConceptScore W1580614654C95444343 @default.
• W1580614654 hasLocation W15806146541 @default.
• W1580614654 hasOpenAccess W1580614654 @default.
• W1580614654 hasPrimaryLocation W15806146541 @default.
• W1580614654 hasRelatedWork W18010411 @default.
• W1580614654 hasRelatedWork W2061548409 @default.
• W1580614654 hasRelatedWork W2137329595 @default.
• W1580614654 hasRelatedWork W2154630337 @default.
• W1580614654 hasRelatedWork W2166278781 @default.
• W1580614654 hasRelatedWork W2167761963 @default.
• W1580614654 hasRelatedWork W2468113650 @default.
• W1580614654 hasRelatedWork W2529922616 @default.
• W1580614654 hasRelatedWork W3030004176 @default.
• W1580614654 hasRelatedWork W3206194141 @default.
• W1580614654 hasVolume "9" @default.
• W1580614654 isParatext "false" @default.
• W1580614654 isRetracted "false" @default.
• W1580614654 magId "1580614654" @default.
• W1580614654 workType "article" @default.