FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1580821441> ?p ?o ?g. }

• W1580821441 endingPage "11408" @default.
• W1580821441 startingPage "11401" @default.
• W1580821441 abstract "Leukocytes use the alpha 6 beta 1 integrin to adhere to laminin based on mAb inhibition and affinity chromatography studies. This adhesion requires leukocyte stimulation with either PMA or specific cytokines, a process that has been termed integrin signaling. In the present study, the involvement of alpha 6 integrin structural variants in this regulated adhesion was examined using mouse macrophages. The two known alpha 6 structural variants, alpha 6A and alpha 6B, differ only in their cytoplasmic domain sequences. Using reverse transcriptase-polymerase chain reaction, we observed that macrophages express only the alpha 6A structural variant, in contrast to most cell types which express both alpha 6A and alpha 6B variants. The role of this integrin subunit in macrophage adhesion was assessed by cDNA transfection of P388D1 cells. We found that this mouse macrophage cell line does not adhere to laminin even in response to phorbol 12-myristate 13-acetate (PMA) stimulation, though it does adhere normally to fibronectin and tissue culture plastic. Subsequent analysis employing reverse transcriptase-polymerase chain reaction and immunoprecipitation of surface labeled cells revealed that this cell line expresses neither the alpha 6A nor alpha 6B integrin subunits. Stable transfection of either the chick or human alpha 6A cDNAs into P388D1 cells resulted in chimeric alpha 6A beta 1 surface expression. The alpha 6A transfectants exhibited inside-out integrin signaling because PMA stimulation markedly increased their ability to adhere to laminin but it did not increase alpha 6A beta 1 surface expression. Similar results were obtained after transfection of the human alpha 6B cDNA. Analysis of the human transfectants was facilitated by the generation of a monoclonal antibody, 2B7, that is specific for the human alpha 6 integrin subunit. These observations demonstrate that both alpha 6A beta 1 and alpha 6B beta 1 can be regulated by inside-out signaling pathways in macrophages, even though this cell type expresses only alpha 6A beta 1. The data presented also demonstrate clearly that the alpha 6A and alpha 6B cytoplasmic domains do not differ in their ability to be regulated by PMA." @default.
• W1580821441 created "2016-06-24" @default.
• W1580821441 creator A5010527699 @default.
• W1580821441 creator A5023136222 @default.
• W1580821441 creator A5091553412 @default.
• W1580821441 date "1993-05-01" @default.
• W1580821441 modified "2023-09-28" @default.
• W1580821441 title "Inside-out integrin signaling in macrophages. Analysis of the role of the alpha 6A beta 1 and alpha 6B beta 1 integrin variants in laminin adhesion by cDNA expression in an alpha 6 integrin-deficient macrophage cell line" @default.
• W1580821441 cites W1594700207 @default.
• W1580821441 doi "https://doi.org/10.1016/s0021-9258(18)82138-x" @default.
• W1580821441 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8496190" @default.
• W1580821441 hasPublicationYear "1993" @default.
• W1580821441 type Work @default.
• W1580821441 sameAs 1580821441 @default.
• W1580821441 citedByCount "81" @default.
• W1580821441 countsByYear W15808214412012 @default.
• W1580821441 countsByYear W15808214412014 @default.
• W1580821441 countsByYear W15808214412018 @default.
• W1580821441 countsByYear W15808214412020 @default.
• W1580821441 countsByYear W15808214412022 @default.
• W1580821441 crossrefType "journal-article" @default.
• W1580821441 hasAuthorship W1580821441A5010527699 @default.
• W1580821441 hasAuthorship W1580821441A5023136222 @default.
• W1580821441 hasAuthorship W1580821441A5091553412 @default.
• W1580821441 hasBestOaLocation W15808214411 @default.
• W1580821441 hasConcept C104317684 @default.
• W1580821441 hasConcept C1491633281 @default.
• W1580821441 hasConcept C153911025 @default.
• W1580821441 hasConcept C156585808 @default.
• W1580821441 hasConcept C159110408 @default.
• W1580821441 hasConcept C164144092 @default.
• W1580821441 hasConcept C171779818 @default.
• W1580821441 hasConcept C185592680 @default.
• W1580821441 hasConcept C187882448 @default.
• W1580821441 hasConcept C189165786 @default.
• W1580821441 hasConcept C195687474 @default.
• W1580821441 hasConcept C199360897 @default.
• W1580821441 hasConcept C2775944032 @default.
• W1580821441 hasConcept C2776174256 @default.
• W1580821441 hasConcept C2777141677 @default.
• W1580821441 hasConcept C2779814568 @default.
• W1580821441 hasConcept C41008148 @default.
• W1580821441 hasConcept C49453240 @default.
• W1580821441 hasConcept C553184892 @default.
• W1580821441 hasConcept C55493867 @default.
• W1580821441 hasConcept C64943373 @default.
• W1580821441 hasConcept C71924100 @default.
• W1580821441 hasConcept C86803240 @default.
• W1580821441 hasConcept C95444343 @default.
• W1580821441 hasConceptScore W1580821441C104317684 @default.
• W1580821441 hasConceptScore W1580821441C1491633281 @default.
• W1580821441 hasConceptScore W1580821441C153911025 @default.
• W1580821441 hasConceptScore W1580821441C156585808 @default.
• W1580821441 hasConceptScore W1580821441C159110408 @default.
• W1580821441 hasConceptScore W1580821441C164144092 @default.
• W1580821441 hasConceptScore W1580821441C171779818 @default.
• W1580821441 hasConceptScore W1580821441C185592680 @default.
• W1580821441 hasConceptScore W1580821441C187882448 @default.
• W1580821441 hasConceptScore W1580821441C189165786 @default.
• W1580821441 hasConceptScore W1580821441C195687474 @default.
• W1580821441 hasConceptScore W1580821441C199360897 @default.
• W1580821441 hasConceptScore W1580821441C2775944032 @default.
• W1580821441 hasConceptScore W1580821441C2776174256 @default.
• W1580821441 hasConceptScore W1580821441C2777141677 @default.
• W1580821441 hasConceptScore W1580821441C2779814568 @default.
• W1580821441 hasConceptScore W1580821441C41008148 @default.
• W1580821441 hasConceptScore W1580821441C49453240 @default.
• W1580821441 hasConceptScore W1580821441C553184892 @default.
• W1580821441 hasConceptScore W1580821441C55493867 @default.
• W1580821441 hasConceptScore W1580821441C64943373 @default.
• W1580821441 hasConceptScore W1580821441C71924100 @default.
• W1580821441 hasConceptScore W1580821441C86803240 @default.
• W1580821441 hasConceptScore W1580821441C95444343 @default.
• W1580821441 hasIssue "15" @default.
• W1580821441 hasLocation W15808214411 @default.
• W1580821441 hasOpenAccess W1580821441 @default.
• W1580821441 hasPrimaryLocation W15808214411 @default.
• W1580821441 hasRelatedWork W1580821441 @default.
• W1580821441 hasRelatedWork W1751145549 @default.
• W1580821441 hasRelatedWork W1939242577 @default.
• W1580821441 hasRelatedWork W1977706672 @default.
• W1580821441 hasRelatedWork W2036258190 @default.
• W1580821441 hasRelatedWork W2051811193 @default.
• W1580821441 hasRelatedWork W2060450254 @default.
• W1580821441 hasRelatedWork W2108283255 @default.
• W1580821441 hasRelatedWork W2981724730 @default.
• W1580821441 hasRelatedWork W4253132586 @default.
• W1580821441 hasVolume "268" @default.
• W1580821441 isParatext "false" @default.
• W1580821441 isRetracted "false" @default.
• W1580821441 magId "1580821441" @default.
• W1580821441 workType "article" @default.