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- W1581203336 abstract "AbstractCanola (Brassica napus) plants with Aster yellows-like symptoms were collected in 2011 and 2012 from canola fields in North Dakota, USA. The presence of phytoplasmas in the symptomatic plants was confirmed by a nested PCR assay using universal primer pairs P1/P7 and R16F2n/R2. Phytoplasmas were identified as ‘Candidatus phytoplasma asteris’ related strains with >99% nucleotide identity to the reference strain M30790 based on in silico analysis of the F2nR2 fragments using the iPhyClassifier online tool. Based on sequence homology searches, RFLP and phylogenetic analyses of 16S rDNA gene, phytoplasmas belonging to two distinct lineages, 16SrI-A and 16SrI-B subgroups, were identified infecting canola in North Dakota. Further characterization of the phytoplasmas based on ribosomal protein operon and secY genes also supported the presence of two distinct lineages. Sequence comparison and RFLP analyses based on the three regions confirmed the presence of mixed infection by phytoplasmas belonging to sub..." @default.
- W1581203336 created "2016-06-24" @default.
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- W1581203336 date "2015-07-03" @default.
- W1581203336 modified "2023-09-24" @default.
- W1581203336 title "Detection and molecular characterization of ‘Candidatusphytoplasma asteris’ related phytoplasmas infecting canola in North Dakota" @default.
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- W1581203336 doi "https://doi.org/10.1080/07060661.2015.1065438" @default.
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