Matches in SemOpenAlex for { <https://semopenalex.org/work/W1581421331> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W1581421331 endingPage "3538" @default.
- W1581421331 startingPage "3532" @default.
- W1581421331 abstract "High affinity receptors for interleukin 2 (IL 2) contain the Tac protein as one ligand-binding subunit. Localization of the IL 2-binding site on this molecule, as well as localization of the complementary site on IL 2, should provide insight into the design of IL 2 analogs. In this report, we examine the ability of normal and modified Tac protein to bind IL 2 and several antibodies that recognize the native Tac molecule. Using a transient L cell expression system, we have determined that transfection with cDNA-missing Tac exon 4 resulted in expression of spliced protein that had no measurable binding to IL 2 or the monoclonal anti-receptor antibodies, anti-Tac, and 7G7/B6. This protein was detected, however, by rabbit polyclonal antibodies prepared against synthetic Tac peptides. Thus, one or more amino acids encoded by exon 4 is important, either for direct ligand contact or for the proper folding of critical segments of the Tac molecule. In addition, insertion of stop codons at a unique restriction enzyme site near the beginning of exon 5 resulted in cellular secretion of truncated Tac molecules that were capable of binding IL 2, anti-Tac, and 7G7/B6. Amino acids encoded by exons 5 to 8 thus play no critical role in IL 2 binding. The ligand association demonstrated for truncated Tac protein produced by exons 2 to 4 should guide attempts to define the IL 2-binding segment of the Tac molecule." @default.
- W1581421331 created "2016-06-24" @default.
- W1581421331 creator A5036641112 @default.
- W1581421331 creator A5037273313 @default.
- W1581421331 creator A5066302782 @default.
- W1581421331 creator A5070917422 @default.
- W1581421331 date "1987-05-15" @default.
- W1581421331 modified "2023-10-18" @default.
- W1581421331 title "Structure function relationships for the IL 2 receptor system. III. Tac protein missing amino acids 102 to 173 (exon 4) is unable to bind IL 2: detection of spliced protein after L cell transfection." @default.
- W1581421331 doi "https://doi.org/10.4049/jimmunol.138.10.3532" @default.
- W1581421331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3033076" @default.
- W1581421331 hasPublicationYear "1987" @default.
- W1581421331 type Work @default.
- W1581421331 sameAs 1581421331 @default.
- W1581421331 citedByCount "15" @default.
- W1581421331 crossrefType "journal-article" @default.
- W1581421331 hasAuthorship W1581421331A5036641112 @default.
- W1581421331 hasAuthorship W1581421331A5037273313 @default.
- W1581421331 hasAuthorship W1581421331A5066302782 @default.
- W1581421331 hasAuthorship W1581421331A5070917422 @default.
- W1581421331 hasBestOaLocation W15814213311 @default.
- W1581421331 hasConcept C104317684 @default.
- W1581421331 hasConcept C107824862 @default.
- W1581421331 hasConcept C153911025 @default.
- W1581421331 hasConcept C159654299 @default.
- W1581421331 hasConcept C170493617 @default.
- W1581421331 hasConcept C36823959 @default.
- W1581421331 hasConcept C515207424 @default.
- W1581421331 hasConcept C54355233 @default.
- W1581421331 hasConcept C55493867 @default.
- W1581421331 hasConcept C86803240 @default.
- W1581421331 hasConcept C956191 @default.
- W1581421331 hasConceptScore W1581421331C104317684 @default.
- W1581421331 hasConceptScore W1581421331C107824862 @default.
- W1581421331 hasConceptScore W1581421331C153911025 @default.
- W1581421331 hasConceptScore W1581421331C159654299 @default.
- W1581421331 hasConceptScore W1581421331C170493617 @default.
- W1581421331 hasConceptScore W1581421331C36823959 @default.
- W1581421331 hasConceptScore W1581421331C515207424 @default.
- W1581421331 hasConceptScore W1581421331C54355233 @default.
- W1581421331 hasConceptScore W1581421331C55493867 @default.
- W1581421331 hasConceptScore W1581421331C86803240 @default.
- W1581421331 hasConceptScore W1581421331C956191 @default.
- W1581421331 hasIssue "10" @default.
- W1581421331 hasLocation W15814213311 @default.
- W1581421331 hasLocation W15814213312 @default.
- W1581421331 hasOpenAccess W1581421331 @default.
- W1581421331 hasPrimaryLocation W15814213311 @default.
- W1581421331 hasRelatedWork W1964288182 @default.
- W1581421331 hasRelatedWork W1968369877 @default.
- W1581421331 hasRelatedWork W1978342924 @default.
- W1581421331 hasRelatedWork W2001677368 @default.
- W1581421331 hasRelatedWork W2037918956 @default.
- W1581421331 hasRelatedWork W2064835625 @default.
- W1581421331 hasRelatedWork W2076771161 @default.
- W1581421331 hasRelatedWork W2078492725 @default.
- W1581421331 hasRelatedWork W2090205230 @default.
- W1581421331 hasRelatedWork W2427998189 @default.
- W1581421331 hasVolume "138" @default.
- W1581421331 isParatext "false" @default.
- W1581421331 isRetracted "false" @default.
- W1581421331 magId "1581421331" @default.
- W1581421331 workType "article" @default.