FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1581749368> ?p ?o ?g. }

• W1581749368 endingPage "1019" @default.
• W1581749368 startingPage "1015" @default.
• W1581749368 abstract "Following exposure of L1210 leukemia cells to antifolates, tetrahydrofolate-dependent purine and pyrimidine biosyntheses are blocked despite the presence of the major portion of tetrahydrofolate cofactors. Previous studies from this laboratory demonstrated that this cannot be due to direct inhibition of thymidylate synthase by dihydrofolate polyglutamates or other endogenous folates and suggested that this phenomenon is due to compartmentation of tetrahydrofolate cofactors unavailable for interconversion and/or oxidation when dihydrofolate reductase activity is abolished by antifolates. The present paper evaluates the possibility that tetrahydrofolate cofactors in subcellular organelles, in particular, mitochondria, are unavailable for oxidation by thymidylate synthase. Paniculate and cytosolic fractions were obtained from L1210 cells following homogenization and differential centrifugation. The crude mitochondrial fraction contained 20.1% of the total folate pool and included 5-formyl-tetrahydrofolate, 10-formyltetrahydrofolate and tetrahydrofolate in proportions similar to intact cells. The cytosolic fraction had an increased proportion of tetrahydrofolate and decreased proportions of 5-formyl- and 10-formyltetrahydrofolate relative to intact cells or the particulate fraction. Exposure of cells to 10 μM trimetrexate for 30 min produced ∼45% interconversion of tetrahydrofolate cofactors to dihydrofolate in the cytosolic fraction, a level much greater than that observed in whole cell extracts (25–30%), but had no effect on folate pools in the crude mitochondrial fraction. These data indicate that subcellular compartmentation accounts, in part, for the failure to oxidize tetrahydrofolate cofactors to dihydrofolate in the presence of antifolate levels that abolish dihydrofolate reductase activity." @default.
• W1581749368 created "2016-06-24" @default.
• W1581749368 creator A5041681996 @default.
• W1581749368 creator A5069416760 @default.
• W1581749368 creator A5071736781 @default.
• W1581749368 date "1991-08-01" @default.
• W1581749368 modified "2023-10-18" @default.
• W1581749368 title "Compartmentation of intracellular folates" @default.
• W1581749368 cites W1177624759 @default.
• W1581749368 cites W1485678634 @default.
• W1581749368 cites W1489457330 @default.
• W1581749368 cites W1491882973 @default.
• W1581749368 cites W1492845448 @default.
• W1581749368 cites W1497790249 @default.
• W1581749368 cites W1524243997 @default.
• W1581749368 cites W1555616833 @default.
• W1581749368 cites W1558402642 @default.
• W1581749368 cites W1607907842 @default.
• W1581749368 cites W1673653510 @default.
• W1581749368 cites W1968447305 @default.
• W1581749368 cites W2009794857 @default.
• W1581749368 cites W2010880776 @default.
• W1581749368 cites W2035842529 @default.
• W1581749368 cites W2039467691 @default.
• W1581749368 cites W2049559428 @default.
• W1581749368 cites W2054843714 @default.
• W1581749368 cites W2056375016 @default.
• W1581749368 cites W2075734681 @default.
• W1581749368 cites W2123509576 @default.
• W1581749368 cites W2203773224 @default.
• W1581749368 cites W2399100769 @default.
• W1581749368 cites W2406837475 @default.
• W1581749368 cites W4232813476 @default.
• W1581749368 cites W4293247451 @default.
• W1581749368 cites W44995231 @default.
• W1581749368 doi "https://doi.org/10.1016/0006-2952(91)90283-b" @default.
• W1581749368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1831361" @default.
• W1581749368 hasPublicationYear "1991" @default.
• W1581749368 type Work @default.
• W1581749368 sameAs 1581749368 @default.
• W1581749368 citedByCount "21" @default.
• W1581749368 countsByYear W15817493682012 @default.
• W1581749368 countsByYear W15817493682014 @default.
• W1581749368 countsByYear W15817493682018 @default.
• W1581749368 crossrefType "journal-article" @default.
• W1581749368 hasAuthorship W1581749368A5041681996 @default.
• W1581749368 hasAuthorship W1581749368A5069416760 @default.
• W1581749368 hasAuthorship W1581749368A5071736781 @default.
• W1581749368 hasConcept C109316439 @default.
• W1581749368 hasConcept C123321153 @default.
• W1581749368 hasConcept C181199279 @default.
• W1581749368 hasConcept C185592680 @default.
• W1581749368 hasConcept C197957613 @default.
• W1581749368 hasConcept C202751555 @default.
• W1581749368 hasConcept C203014093 @default.
• W1581749368 hasConcept C2776694085 @default.
• W1581749368 hasConcept C2778397455 @default.
• W1581749368 hasConcept C2780017796 @default.
• W1581749368 hasConcept C2780456651 @default.
• W1581749368 hasConcept C2781059491 @default.
• W1581749368 hasConcept C2781320022 @default.
• W1581749368 hasConcept C2781382166 @default.
• W1581749368 hasConcept C28859421 @default.
• W1581749368 hasConcept C54355233 @default.
• W1581749368 hasConcept C55493867 @default.
• W1581749368 hasConcept C79879829 @default.
• W1581749368 hasConcept C86803240 @default.
• W1581749368 hasConcept C98539663 @default.
• W1581749368 hasConceptScore W1581749368C109316439 @default.
• W1581749368 hasConceptScore W1581749368C123321153 @default.
• W1581749368 hasConceptScore W1581749368C181199279 @default.
• W1581749368 hasConceptScore W1581749368C185592680 @default.
• W1581749368 hasConceptScore W1581749368C197957613 @default.
• W1581749368 hasConceptScore W1581749368C202751555 @default.
• W1581749368 hasConceptScore W1581749368C203014093 @default.
• W1581749368 hasConceptScore W1581749368C2776694085 @default.
• W1581749368 hasConceptScore W1581749368C2778397455 @default.
• W1581749368 hasConceptScore W1581749368C2780017796 @default.
• W1581749368 hasConceptScore W1581749368C2780456651 @default.
• W1581749368 hasConceptScore W1581749368C2781059491 @default.
• W1581749368 hasConceptScore W1581749368C2781320022 @default.
• W1581749368 hasConceptScore W1581749368C2781382166 @default.
• W1581749368 hasConceptScore W1581749368C28859421 @default.
• W1581749368 hasConceptScore W1581749368C54355233 @default.
• W1581749368 hasConceptScore W1581749368C55493867 @default.
• W1581749368 hasConceptScore W1581749368C79879829 @default.
• W1581749368 hasConceptScore W1581749368C86803240 @default.
• W1581749368 hasConceptScore W1581749368C98539663 @default.
• W1581749368 hasIssue "5" @default.
• W1581749368 hasLocation W15817493681 @default.
• W1581749368 hasLocation W15817493682 @default.
• W1581749368 hasOpenAccess W1581749368 @default.
• W1581749368 hasPrimaryLocation W15817493681 @default.
• W1581749368 hasRelatedWork W1504906650 @default.
• W1581749368 hasRelatedWork W1536608791 @default.
• W1581749368 hasRelatedWork W2005962086 @default.
• W1581749368 hasRelatedWork W2020736105 @default.
• W1581749368 hasRelatedWork W2026018236 @default.

• next