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- W1582489284 abstract "Trypanosoma brucei is a unicellular parasite causing African sleeping sickness in cattle and humans. Due to the ease with which these cells can be cultured and genetically manipulated, it has emerged as a model organism for the kinetoplastids.In this chapter we describe the preparation of T. brucei for transmission electron microscopy. A thorough explanation of conventional sample preparation through chemical fixation of whole cells and detergent extracted cytoskeletons followed by dehydration and Epon embedding is given. We also introduce a novel high-pressure freezing protocol, which followed by rapid freeze substitution and HM20 embedding generates T. brucei samples displaying good cell morphology, which are suitable for immunocytochemistry." @default.
- W1582489284 created "2016-06-24" @default.
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- W1582489284 date "2010-01-01" @default.
- W1582489284 modified "2023-10-16" @default.
- W1582489284 title "Ultrastructural Investigation Methods for Trypanosoma brucei" @default.
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- W1582489284 doi "https://doi.org/10.1016/s0091-679x(10)96008-1" @default.
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