FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1582531808> ?p ?o ?g. }

• W1582531808 endingPage "11875" @default.
• W1582531808 startingPage "11871" @default.
• W1582531808 abstract "Small mammals, including human infants, rely on nonshivering thermogenesis for a substantial portion of their body heat during exposure to cold. This thermogenesis is mediated in large part by the uncoupling protein, which is found exclusively within the inner membrane of brown adipose tissue mitochondria. The sole function of uncoupling protein is to provide a regulated transport pathway for electrophoretic back-flux of H+ ions into the mitochondrial matrix, thereby dissipating the protonmotive force and producing heat. Thus, uncoupling protein is unique with respect to both its physiological role and its tissue expression. We have now achieved high level expression of rat uncoupling protein in yeast, with import into yeast mitochondria at levels, 70-100 micrograms/mg of mitochondrial protein, similar to those observed in brown adipose tissue mitochondria from cold-adapted rats. When the expressed protein was purified and reconstituted into liposomes, the proteoliposomes exhibited GDP-sensitive proton and chloride uniports that were inhibited by GDP with Ki values similar to those obtained with native protein. Moreover, the molecular activities of the expressed protein with respect to Cl- and H+ transport were indistinguishable from those of native protein. The availability of unlimited amounts of functional, expressed uncoupling protein will now permit application of site-directed mutagenesis to the many intriguing aspects of uncoupling protein structure and function." @default.
• W1582531808 created "2016-06-24" @default.
• W1582531808 creator A5004430188 @default.
• W1582531808 creator A5016077843 @default.
• W1582531808 creator A5017880433 @default.
• W1582531808 creator A5026769141 @default.
• W1582531808 creator A5052024341 @default.
• W1582531808 creator A5057936788 @default.
• W1582531808 date "1991-06-01" @default.
• W1582531808 modified "2023-10-10" @default.
• W1582531808 title "Functional reconstitution of rat uncoupling protein following its high level expression in yeast" @default.
• W1582531808 cites W1012540603 @default.
• W1582531808 cites W1152005457 @default.
• W1582531808 cites W1437027770 @default.
• W1582531808 cites W1502128823 @default.
• W1582531808 cites W1507851645 @default.
• W1582531808 cites W1518847852 @default.
• W1582531808 cites W1519247927 @default.
• W1582531808 cites W1523078511 @default.
• W1582531808 cites W1554331870 @default.
• W1582531808 cites W1560402341 @default.
• W1582531808 cites W1564572504 @default.
• W1582531808 cites W1582080277 @default.
• W1582531808 cites W1607064730 @default.
• W1582531808 cites W1969890353 @default.
• W1582531808 cites W1975323382 @default.
• W1582531808 cites W1978888607 @default.
• W1582531808 cites W1996659589 @default.
• W1582531808 cites W2016486124 @default.
• W1582531808 cites W2016936385 @default.
• W1582531808 cites W2016947142 @default.
• W1582531808 cites W2040780517 @default.
• W1582531808 cites W2051043527 @default.
• W1582531808 cites W2051047276 @default.
• W1582531808 cites W2053732193 @default.
• W1582531808 cites W2070342467 @default.
• W1582531808 cites W2082767273 @default.
• W1582531808 cites W2083265258 @default.
• W1582531808 cites W2086198406 @default.
• W1582531808 cites W2095500810 @default.
• W1582531808 cites W2097622664 @default.
• W1582531808 cites W2134887033 @default.
• W1582531808 cites W2165447592 @default.
• W1582531808 cites W331117150 @default.
• W1582531808 doi "https://doi.org/10.1016/s0021-9258(18)99038-1" @default.
• W1582531808 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2050682" @default.
• W1582531808 hasPublicationYear "1991" @default.
• W1582531808 type Work @default.
• W1582531808 sameAs 1582531808 @default.
• W1582531808 citedByCount "55" @default.
• W1582531808 countsByYear W15825318082014 @default.
• W1582531808 countsByYear W15825318082017 @default.
• W1582531808 countsByYear W15825318082021 @default.
• W1582531808 countsByYear W15825318082023 @default.
• W1582531808 crossrefType "journal-article" @default.
• W1582531808 hasAuthorship W1582531808A5004430188 @default.
• W1582531808 hasAuthorship W1582531808A5016077843 @default.
• W1582531808 hasAuthorship W1582531808A5017880433 @default.
• W1582531808 hasAuthorship W1582531808A5026769141 @default.
• W1582531808 hasAuthorship W1582531808A5052024341 @default.
• W1582531808 hasAuthorship W1582531808A5057936788 @default.
• W1582531808 hasBestOaLocation W15825318081 @default.
• W1582531808 hasConcept C104317684 @default.
• W1582531808 hasConcept C171089720 @default.
• W1582531808 hasConcept C185592680 @default.
• W1582531808 hasConcept C199360897 @default.
• W1582531808 hasConcept C2779222958 @default.
• W1582531808 hasConcept C2779406938 @default.
• W1582531808 hasConcept C2780642333 @default.
• W1582531808 hasConcept C41008148 @default.
• W1582531808 hasConcept C55493867 @default.
• W1582531808 hasConcept C6856738 @default.
• W1582531808 hasConcept C86803240 @default.
• W1582531808 hasConcept C90559484 @default.
• W1582531808 hasConcept C95444343 @default.
• W1582531808 hasConceptScore W1582531808C104317684 @default.
• W1582531808 hasConceptScore W1582531808C171089720 @default.
• W1582531808 hasConceptScore W1582531808C185592680 @default.
• W1582531808 hasConceptScore W1582531808C199360897 @default.
• W1582531808 hasConceptScore W1582531808C2779222958 @default.
• W1582531808 hasConceptScore W1582531808C2779406938 @default.
• W1582531808 hasConceptScore W1582531808C2780642333 @default.
• W1582531808 hasConceptScore W1582531808C41008148 @default.
• W1582531808 hasConceptScore W1582531808C55493867 @default.
• W1582531808 hasConceptScore W1582531808C6856738 @default.
• W1582531808 hasConceptScore W1582531808C86803240 @default.
• W1582531808 hasConceptScore W1582531808C90559484 @default.
• W1582531808 hasConceptScore W1582531808C95444343 @default.
• W1582531808 hasIssue "18" @default.
• W1582531808 hasLocation W15825318081 @default.
• W1582531808 hasOpenAccess W1582531808 @default.
• W1582531808 hasPrimaryLocation W15825318081 @default.
• W1582531808 hasRelatedWork W1274460528 @default.
• W1582531808 hasRelatedWork W1972962979 @default.
• W1582531808 hasRelatedWork W1978776261 @default.
• W1582531808 hasRelatedWork W2025917340 @default.
• W1582531808 hasRelatedWork W2057651775 @default.
• W1582531808 hasRelatedWork W2062385823 @default.

• next