Matches in SemOpenAlex for { <https://semopenalex.org/work/W1583462687> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W1583462687 endingPage "8189" @default.
- W1583462687 startingPage "8183" @default.
- W1583462687 abstract "Abstract The decomposition of the phosphorylated intermediate (EP) of sarcoplasmic reticulum ATPase, purified by the method of deoxycholic acid extraction, was studied by first phosphorylating with [gamma-32P]ATP, then diluting the reaction mixture with 20 volumes of medium containing nonradioactive ATP, and finally quenching serial samples with acid for determination of residual [32P]EP. The time course of [32P]EP decomposition consists of an initial fast phase followed by a slow phase. The two components of EP, EPfast (1.1 nmol/mg) and EPslow (2.8 nmol/mg), decomposed with the rate constants of 6 and 0.8 min-1, respectively, in the presence of 0.5 mM CaCl2, 5mM MgCl2, and 90 mM KCl at pH 7.0 and O degrees C. The sum of the hydrolytic activities corresponding to the two components accounts for the steady state velocity of the Pi production under the same conditions, indicating that the two components represent simultaneous pathways, rather than sequential steps of EP decomposition. As the time of phosphorylation with [gamma-32P]ATP is increased from 2 to 15 s, the fraction of EPfast decreases in favor of EPslow. This conversion decreases the rate of total Pi production by the enzyme following an initial Pi burst. Conversion of EPfast to EPslow is favored by millimolar concentrations of Ca2+. On the other hand, conversion of EPslow to EPfast is obtained by reducing Ca2+ or raising Mg2+ concentration, but is prevented by removal of ADP. The EPslow fraction decreases in favor of EPfast as the temperature is increased from 0 to 22 degrees C." @default.
- W1583462687 created "2016-06-24" @default.
- W1583462687 creator A5029731768 @default.
- W1583462687 date "1984-07-01" @default.
- W1583462687 modified "2023-09-29" @default.
- W1583462687 title "Two alternate kinetic routes for the decomposition of the phosphorylated intermediate of sarcoplasmic reticulum Ca2+-ATPase." @default.
- W1583462687 cites W147967219 @default.
- W1583462687 cites W1545235916 @default.
- W1583462687 cites W1599854046 @default.
- W1583462687 cites W1602586459 @default.
- W1583462687 cites W1618664245 @default.
- W1583462687 cites W1737644385 @default.
- W1583462687 cites W1807150379 @default.
- W1583462687 cites W2002885745 @default.
- W1583462687 cites W2005826266 @default.
- W1583462687 cites W2041683725 @default.
- W1583462687 cites W2070838650 @default.
- W1583462687 cites W2099743460 @default.
- W1583462687 cites W2109366031 @default.
- W1583462687 cites W2114439837 @default.
- W1583462687 cites W2143677602 @default.
- W1583462687 cites W2299086764 @default.
- W1583462687 cites W2418606782 @default.
- W1583462687 cites W365803441 @default.
- W1583462687 cites W66338719 @default.
- W1583462687 doi "https://doi.org/10.1016/s0021-9258(17)39711-9" @default.
- W1583462687 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6234309" @default.
- W1583462687 hasPublicationYear "1984" @default.
- W1583462687 type Work @default.
- W1583462687 sameAs 1583462687 @default.
- W1583462687 citedByCount "21" @default.
- W1583462687 countsByYear W15834626872014 @default.
- W1583462687 countsByYear W15834626872015 @default.
- W1583462687 countsByYear W15834626872016 @default.
- W1583462687 crossrefType "journal-article" @default.
- W1583462687 hasAuthorship W1583462687A5029731768 @default.
- W1583462687 hasBestOaLocation W15834626871 @default.
- W1583462687 hasConcept C101571972 @default.
- W1583462687 hasConcept C11960822 @default.
- W1583462687 hasConcept C121332964 @default.
- W1583462687 hasConcept C124681953 @default.
- W1583462687 hasConcept C12554922 @default.
- W1583462687 hasConcept C135889238 @default.
- W1583462687 hasConcept C158617107 @default.
- W1583462687 hasConcept C178790620 @default.
- W1583462687 hasConcept C181199279 @default.
- W1583462687 hasConcept C185592680 @default.
- W1583462687 hasConcept C23265538 @default.
- W1583462687 hasConcept C55493867 @default.
- W1583462687 hasConcept C62520636 @default.
- W1583462687 hasConcept C86803240 @default.
- W1583462687 hasConcept C95444343 @default.
- W1583462687 hasConceptScore W1583462687C101571972 @default.
- W1583462687 hasConceptScore W1583462687C11960822 @default.
- W1583462687 hasConceptScore W1583462687C121332964 @default.
- W1583462687 hasConceptScore W1583462687C124681953 @default.
- W1583462687 hasConceptScore W1583462687C12554922 @default.
- W1583462687 hasConceptScore W1583462687C135889238 @default.
- W1583462687 hasConceptScore W1583462687C158617107 @default.
- W1583462687 hasConceptScore W1583462687C178790620 @default.
- W1583462687 hasConceptScore W1583462687C181199279 @default.
- W1583462687 hasConceptScore W1583462687C185592680 @default.
- W1583462687 hasConceptScore W1583462687C23265538 @default.
- W1583462687 hasConceptScore W1583462687C55493867 @default.
- W1583462687 hasConceptScore W1583462687C62520636 @default.
- W1583462687 hasConceptScore W1583462687C86803240 @default.
- W1583462687 hasConceptScore W1583462687C95444343 @default.
- W1583462687 hasIssue "13" @default.
- W1583462687 hasLocation W15834626871 @default.
- W1583462687 hasOpenAccess W1583462687 @default.
- W1583462687 hasPrimaryLocation W15834626871 @default.
- W1583462687 hasRelatedWork W1155355392 @default.
- W1583462687 hasRelatedWork W1528876560 @default.
- W1583462687 hasRelatedWork W1576186528 @default.
- W1583462687 hasRelatedWork W1967311801 @default.
- W1583462687 hasRelatedWork W1984052139 @default.
- W1583462687 hasRelatedWork W2018102701 @default.
- W1583462687 hasRelatedWork W2056985152 @default.
- W1583462687 hasRelatedWork W2403275950 @default.
- W1583462687 hasRelatedWork W2436268350 @default.
- W1583462687 hasRelatedWork W2463375115 @default.
- W1583462687 hasVolume "259" @default.
- W1583462687 isParatext "false" @default.
- W1583462687 isRetracted "false" @default.
- W1583462687 magId "1583462687" @default.
- W1583462687 workType "article" @default.