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- W158351495 abstract "From the Friend virus complex, the helper-independent component, F-MuLV, and the replication-defective component, SFFV, have been biologically cloned, and subsequently molecularly cloned in bacterial vectors. The molecularly cloned forms of each virus are infectious in gene transfer studies. F-MuLV induces an erythropoietic proliferative disease with anemia in newborn Swiss mice, while SFFV determines a polycythemic erythroblastosis in adult mice. By molecular cloning techniques, we have identified that the 3′ 3.1 Kb of F-MuLV carries the disease-inducing gene(s) of F-MuLV. Cell culture conditions have been developed which allow long-term erythropoiesis in Dexter bone-marrow cultures, and effects of F-MuLV and of SFFV have been observed in this culture system. The results suggest for the first time a cell culture assay for the growth promoting effects on hematopoietic cells of helper-independent type-C leukemia viruses." @default.
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- W158351495 date "1980-01-01" @default.
- W158351495 modified "2023-09-28" @default.
- W158351495 title "MOLECULAR GENETICS AND CELL CULTURE ASSAYS FOR HELPER-INDEPENDENT AND REPLICATION-DEFECTIVE COMPONENTS OF THE FRIEND VIRUS COMPLEX" @default.
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- W158351495 doi "https://doi.org/10.1016/b978-0-12-255850-4.50042-1" @default.
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