Matches in SemOpenAlex for { <https://semopenalex.org/work/W1583733441> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1583733441 endingPage "491" @default.
- W1583733441 startingPage "477" @default.
- W1583733441 abstract "Ribonucleoprotein particles isolated from extracts of vesicular stomatitis virus (VSV) -infected L cells synthesized in vitro four classes of polyadenylated RNA sedimenting at 29S, 19S, 17S, and 13S. When synthesized in vitro in the presence of the methyl donor S-adenosyl methionine, these RNA species contained the following 5'-terminal structures: (i) m7G5ppp5'AmpAp(70%) ; (ii) m7G5'ppp5'AmpAmpNp (20%) and (iii) pppAp (10%). In the presence of the methylation inhibitor S-adenosylhomocysteine, however, the mRNA contained the 5'-terminal structures G5'ppp5'Ap (80%) and pppAp (20%). The mRNA's synthesized in vitro were translated in the homologous ascites and the heterologous wheat embryo cell-free systems. In both, the products were shown by sodium dodecyl sulfate gel electrophoresis and by immunoprecipitation to contain all five viral proteins, L, G, N, NS, and M. The presumed precursor to the G protein (G*) was also identified by fingerprint analysis. Methylated VSV mRNA was more active in protein synthesis than unmethylated mRNA in both the ascites system and the wheat embryo systems. Addition of S-adenosylmethionine stimulated translation of unmethylated mRNA in the wheat embryo but not in the ascites extract. S-adenosylhomocysteine, however, by preventing mRNA methylation inhibited the translation of unmethylated VSV mRNA in both systems. The mRNA methylating activity present in wheat embryo S-30 extracts was recovered in the ribosome-free supernatant fraction (S-150) and was insensitive to the protein synthesis inhibitor pactamycin." @default.
- W1583733441 created "2016-06-24" @default.
- W1583733441 creator A5072700846 @default.
- W1583733441 creator A5073168603 @default.
- W1583733441 date "1976-02-01" @default.
- W1583733441 modified "2023-09-26" @default.
- W1583733441 title "Characterization and translation of methylated and unmethylated vesicular stomatitis virus mRNA synthesized in vitro by ribonucleoprotein particles from vesicular stomatitis virus-infected L cells" @default.
- W1583733441 cites W1544760751 @default.
- W1583733441 cites W1556158097 @default.
- W1583733441 cites W1569855704 @default.
- W1583733441 cites W1572244763 @default.
- W1583733441 cites W1578428334 @default.
- W1583733441 cites W1584168065 @default.
- W1583733441 cites W1769491152 @default.
- W1583733441 cites W1858188475 @default.
- W1583733441 cites W1969817011 @default.
- W1583733441 cites W1972398797 @default.
- W1583733441 cites W1972635127 @default.
- W1583733441 cites W1975751316 @default.
- W1583733441 cites W1982862778 @default.
- W1583733441 cites W1985517822 @default.
- W1583733441 cites W2006193939 @default.
- W1583733441 cites W2018289835 @default.
- W1583733441 cites W2023675771 @default.
- W1583733441 cites W2023774002 @default.
- W1583733441 cites W2025091176 @default.
- W1583733441 cites W2028385538 @default.
- W1583733441 cites W2040795551 @default.
- W1583733441 cites W2041934807 @default.
- W1583733441 cites W2045281368 @default.
- W1583733441 cites W2066160869 @default.
- W1583733441 cites W2072644415 @default.
- W1583733441 cites W2082221845 @default.
- W1583733441 cites W2093040509 @default.
- W1583733441 cites W2100837269 @default.
- W1583733441 cites W2132356487 @default.
- W1583733441 cites W2164273062 @default.
- W1583733441 cites W3119588676 @default.
- W1583733441 doi "https://doi.org/10.1128/jvi.17.2.477-491.1976" @default.
- W1583733441 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/515440" @default.
- W1583733441 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/176426" @default.
- W1583733441 hasPublicationYear "1976" @default.
- W1583733441 type Work @default.
- W1583733441 sameAs 1583733441 @default.
- W1583733441 citedByCount "48" @default.
- W1583733441 countsByYear W15837334412013 @default.
- W1583733441 crossrefType "journal-article" @default.
- W1583733441 hasAuthorship W1583733441A5072700846 @default.
- W1583733441 hasAuthorship W1583733441A5073168603 @default.
- W1583733441 hasBestOaLocation W15837334411 @default.
- W1583733441 hasConcept C104317684 @default.
- W1583733441 hasConcept C105580179 @default.
- W1583733441 hasConcept C153911025 @default.
- W1583733441 hasConcept C159047783 @default.
- W1583733441 hasConcept C2522874641 @default.
- W1583733441 hasConcept C2779057330 @default.
- W1583733441 hasConcept C3675279 @default.
- W1583733441 hasConcept C55493867 @default.
- W1583733441 hasConcept C65666806 @default.
- W1583733441 hasConcept C67705224 @default.
- W1583733441 hasConcept C75934600 @default.
- W1583733441 hasConcept C86803240 @default.
- W1583733441 hasConcept C88478588 @default.
- W1583733441 hasConceptScore W1583733441C104317684 @default.
- W1583733441 hasConceptScore W1583733441C105580179 @default.
- W1583733441 hasConceptScore W1583733441C153911025 @default.
- W1583733441 hasConceptScore W1583733441C159047783 @default.
- W1583733441 hasConceptScore W1583733441C2522874641 @default.
- W1583733441 hasConceptScore W1583733441C2779057330 @default.
- W1583733441 hasConceptScore W1583733441C3675279 @default.
- W1583733441 hasConceptScore W1583733441C55493867 @default.
- W1583733441 hasConceptScore W1583733441C65666806 @default.
- W1583733441 hasConceptScore W1583733441C67705224 @default.
- W1583733441 hasConceptScore W1583733441C75934600 @default.
- W1583733441 hasConceptScore W1583733441C86803240 @default.
- W1583733441 hasConceptScore W1583733441C88478588 @default.
- W1583733441 hasIssue "2" @default.
- W1583733441 hasLocation W15837334411 @default.
- W1583733441 hasLocation W15837334412 @default.
- W1583733441 hasLocation W15837334413 @default.
- W1583733441 hasOpenAccess W1583733441 @default.
- W1583733441 hasPrimaryLocation W15837334411 @default.
- W1583733441 hasRelatedWork W1543875753 @default.
- W1583733441 hasRelatedWork W1638218691 @default.
- W1583733441 hasRelatedWork W2060980132 @default.
- W1583733441 hasRelatedWork W2081714384 @default.
- W1583733441 hasRelatedWork W2089876360 @default.
- W1583733441 hasRelatedWork W2150075316 @default.
- W1583733441 hasRelatedWork W2408828985 @default.
- W1583733441 hasRelatedWork W2411477978 @default.
- W1583733441 hasRelatedWork W4233898463 @default.
- W1583733441 hasRelatedWork W53065846 @default.
- W1583733441 hasVolume "17" @default.
- W1583733441 isParatext "false" @default.
- W1583733441 isRetracted "false" @default.
- W1583733441 magId "1583733441" @default.
- W1583733441 workType "article" @default.