Matches in SemOpenAlex for { <https://semopenalex.org/work/W1584328291> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1584328291 endingPage "387" @default.
- W1584328291 startingPage "373" @default.
- W1584328291 abstract "The stable maintenance of the unit-copy λ-P1:5R miniplasmid is dependent on adjacent but separable replication (rep) and partition (par) regions of DNA derived from its P1 plasmid parent. The par region consists of an approximately 2·5×103 base-pair (kb) segment of DNA of which the terminal kb contains the plasmid incompatibility determinant incB. Two of the 14 λ-P1:5R partition-defective point mutants isolated are amber (nonsense) mutants, showing that a plasmid-encoded protein is essential for proper partition. All of the Par− point mutants are complemented by the wild-type par region in trans. The complementing activity was shown to be an Mr 44,000 protein endoced by the end of the par region distal to incB. Delection analysis showed that the incB sequence is essential in cis to the plasmid in order, that the plasmid be receptive to the par protein. Thus incB appears to be the target site for par protein activity. We propose that the protein binds to incB, forming a complex that is recognized as a substrate for the cellular partition apparatus. The ability of a cloned incB sequence to compete for the par protein or for the cellular partition apparatus accounts for its activity as an incompatibility determinant. The existence of a plasmid-endoced par protein suggests a specific model for equipartition." @default.
- W1584328291 created "2016-06-24" @default.
- W1584328291 creator A5051020752 @default.
- W1584328291 creator A5062457036 @default.
- W1584328291 date "1983-09-01" @default.
- W1584328291 modified "2023-09-25" @default.
- W1584328291 title "Partition of unit-copy miniplasmids to daughter cells" @default.
- W1584328291 cites W1492813001 @default.
- W1584328291 cites W1499831384 @default.
- W1584328291 cites W1532579456 @default.
- W1584328291 cites W1557830462 @default.
- W1584328291 cites W1584586118 @default.
- W1584328291 cites W1586539673 @default.
- W1584328291 cites W1973149649 @default.
- W1584328291 cites W1998022172 @default.
- W1584328291 cites W2002411289 @default.
- W1584328291 cites W2003037900 @default.
- W1584328291 cites W2008897847 @default.
- W1584328291 cites W2030941519 @default.
- W1584328291 cites W2041003602 @default.
- W1584328291 cites W2070431949 @default.
- W1584328291 cites W2075777344 @default.
- W1584328291 cites W2166153420 @default.
- W1584328291 cites W2350650972 @default.
- W1584328291 cites W4230389869 @default.
- W1584328291 cites W4296765338 @default.
- W1584328291 doi "https://doi.org/10.1016/s0022-2836(83)80056-4" @default.
- W1584328291 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6225875" @default.
- W1584328291 hasPublicationYear "1983" @default.
- W1584328291 type Work @default.
- W1584328291 sameAs 1584328291 @default.
- W1584328291 citedByCount "117" @default.
- W1584328291 countsByYear W15843282912012 @default.
- W1584328291 countsByYear W15843282912014 @default.
- W1584328291 countsByYear W15843282912015 @default.
- W1584328291 countsByYear W15843282912019 @default.
- W1584328291 countsByYear W15843282912020 @default.
- W1584328291 countsByYear W15843282912022 @default.
- W1584328291 countsByYear W15843282912023 @default.
- W1584328291 crossrefType "journal-article" @default.
- W1584328291 hasAuthorship W1584328291A5051020752 @default.
- W1584328291 hasAuthorship W1584328291A5062457036 @default.
- W1584328291 hasConcept C104317684 @default.
- W1584328291 hasConcept C143065580 @default.
- W1584328291 hasConcept C153911025 @default.
- W1584328291 hasConcept C22744801 @default.
- W1584328291 hasConcept C54355233 @default.
- W1584328291 hasConcept C552990157 @default.
- W1584328291 hasConcept C86803240 @default.
- W1584328291 hasConceptScore W1584328291C104317684 @default.
- W1584328291 hasConceptScore W1584328291C143065580 @default.
- W1584328291 hasConceptScore W1584328291C153911025 @default.
- W1584328291 hasConceptScore W1584328291C22744801 @default.
- W1584328291 hasConceptScore W1584328291C54355233 @default.
- W1584328291 hasConceptScore W1584328291C552990157 @default.
- W1584328291 hasConceptScore W1584328291C86803240 @default.
- W1584328291 hasIssue "2" @default.
- W1584328291 hasLocation W15843282911 @default.
- W1584328291 hasLocation W15843282912 @default.
- W1584328291 hasOpenAccess W1584328291 @default.
- W1584328291 hasPrimaryLocation W15843282911 @default.
- W1584328291 hasRelatedWork W106631345 @default.
- W1584328291 hasRelatedWork W171314918 @default.
- W1584328291 hasRelatedWork W1963976589 @default.
- W1584328291 hasRelatedWork W1984671670 @default.
- W1584328291 hasRelatedWork W2002128513 @default.
- W1584328291 hasRelatedWork W2048188137 @default.
- W1584328291 hasRelatedWork W2048658178 @default.
- W1584328291 hasRelatedWork W2095241065 @default.
- W1584328291 hasRelatedWork W2150914257 @default.
- W1584328291 hasRelatedWork W2156674132 @default.
- W1584328291 hasVolume "169" @default.
- W1584328291 isParatext "false" @default.
- W1584328291 isRetracted "false" @default.
- W1584328291 magId "1584328291" @default.
- W1584328291 workType "article" @default.