Matches in SemOpenAlex for { <https://semopenalex.org/work/W1584328685> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1584328685 endingPage "4763" @default.
- W1584328685 startingPage "4758" @default.
- W1584328685 abstract "Expression vectors containing full-length cDNAs for each of the human fibrinogen chains were constructed. COS-1 cells were transfected with single vectors, mixtures of two, or with all three vectors and stable cell lines selected. Cells transfected with single vectors, or with mixtures of any two vectors, expressed the appropriate fibrinogen chains but did not secrete them. COS cells transfected with three vectors expressed all of the chains and secreted fibrinogen. COS cells transfected with three vectors contained, intracellularly, a mixture of fibrinogen-related proteins. The four main intracellular products were nascent fibrinogen, an A alpha.gamma complex, free A alpha chains, and free gamma chains. This is a similar pattern to that noted in Hep G2 cells. The intracellular forms of fibrinogen were sensitive to endoglycosidase H, indicating that they reside in a pre-Golgi compartment. Secreted fibrinogen was endoglycosidase H-insensitive, suggesting that the secreted glycoprotein moieties were processed in the normal manner. When mixed with plasma fibrinogen, radiolabeled recombinant fibrinogen was incorporated into a thrombin-induced clot. These studies demonstrate that COS cells transfected with all three fibrinogen chain cDNAs are capable of assembling and secreting a functional fibrinogen molecule." @default.
- W1584328685 created "2016-06-24" @default.
- W1584328685 creator A5013204133 @default.
- W1584328685 creator A5019523745 @default.
- W1584328685 creator A5032656628 @default.
- W1584328685 creator A5085940021 @default.
- W1584328685 date "1991-03-01" @default.
- W1584328685 modified "2023-09-27" @default.
- W1584328685 title "Assembly and secretion of recombinant human fibrinogen." @default.
- W1584328685 cites W1528432262 @default.
- W1584328685 cites W1552492783 @default.
- W1584328685 cites W1557524780 @default.
- W1584328685 cites W1574905950 @default.
- W1584328685 cites W1577941007 @default.
- W1584328685 cites W1963731813 @default.
- W1584328685 cites W1965372133 @default.
- W1584328685 cites W1968056160 @default.
- W1584328685 cites W1975826484 @default.
- W1584328685 cites W1976981613 @default.
- W1584328685 cites W1983169678 @default.
- W1584328685 cites W1997463088 @default.
- W1584328685 cites W2000908780 @default.
- W1584328685 cites W2013753598 @default.
- W1584328685 cites W2016574785 @default.
- W1584328685 cites W2021898682 @default.
- W1584328685 cites W2023183303 @default.
- W1584328685 cites W2031119708 @default.
- W1584328685 cites W2033691134 @default.
- W1584328685 cites W2037431261 @default.
- W1584328685 cites W2049672602 @default.
- W1584328685 cites W2051248831 @default.
- W1584328685 cites W2062638551 @default.
- W1584328685 cites W2069112575 @default.
- W1584328685 cites W2095331571 @default.
- W1584328685 cites W2135846300 @default.
- W1584328685 cites W2152708819 @default.
- W1584328685 cites W2162336716 @default.
- W1584328685 cites W2172683288 @default.
- W1584328685 cites W2179803393 @default.
- W1584328685 cites W2292067454 @default.
- W1584328685 cites W4230153673 @default.
- W1584328685 cites W59784046 @default.
- W1584328685 doi "https://doi.org/10.1016/s0021-9258(19)67714-8" @default.
- W1584328685 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2002025" @default.
- W1584328685 hasPublicationYear "1991" @default.
- W1584328685 type Work @default.
- W1584328685 sameAs 1584328685 @default.
- W1584328685 citedByCount "60" @default.
- W1584328685 countsByYear W15843286852014 @default.
- W1584328685 countsByYear W15843286852015 @default.
- W1584328685 countsByYear W15843286852019 @default.
- W1584328685 countsByYear W15843286852020 @default.
- W1584328685 crossrefType "journal-article" @default.
- W1584328685 hasAuthorship W1584328685A5013204133 @default.
- W1584328685 hasAuthorship W1584328685A5019523745 @default.
- W1584328685 hasAuthorship W1584328685A5032656628 @default.
- W1584328685 hasAuthorship W1584328685A5085940021 @default.
- W1584328685 hasBestOaLocation W15843286851 @default.
- W1584328685 hasConcept C104317684 @default.
- W1584328685 hasConcept C185592680 @default.
- W1584328685 hasConcept C2779036427 @default.
- W1584328685 hasConcept C40767141 @default.
- W1584328685 hasConcept C49039625 @default.
- W1584328685 hasConcept C55493867 @default.
- W1584328685 hasConcept C70721500 @default.
- W1584328685 hasConcept C86803240 @default.
- W1584328685 hasConceptScore W1584328685C104317684 @default.
- W1584328685 hasConceptScore W1584328685C185592680 @default.
- W1584328685 hasConceptScore W1584328685C2779036427 @default.
- W1584328685 hasConceptScore W1584328685C40767141 @default.
- W1584328685 hasConceptScore W1584328685C49039625 @default.
- W1584328685 hasConceptScore W1584328685C55493867 @default.
- W1584328685 hasConceptScore W1584328685C70721500 @default.
- W1584328685 hasConceptScore W1584328685C86803240 @default.
- W1584328685 hasIssue "8" @default.
- W1584328685 hasLocation W15843286851 @default.
- W1584328685 hasOpenAccess W1584328685 @default.
- W1584328685 hasPrimaryLocation W15843286851 @default.
- W1584328685 hasRelatedWork W1963839712 @default.
- W1584328685 hasRelatedWork W2030904449 @default.
- W1584328685 hasRelatedWork W2049694601 @default.
- W1584328685 hasRelatedWork W2110721047 @default.
- W1584328685 hasRelatedWork W2128025835 @default.
- W1584328685 hasRelatedWork W2267751458 @default.
- W1584328685 hasRelatedWork W2354386436 @default.
- W1584328685 hasRelatedWork W2388691744 @default.
- W1584328685 hasRelatedWork W2734207448 @default.
- W1584328685 hasRelatedWork W2794306218 @default.
- W1584328685 hasVolume "266" @default.
- W1584328685 isParatext "false" @default.
- W1584328685 isRetracted "false" @default.
- W1584328685 magId "1584328685" @default.
- W1584328685 workType "article" @default.