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- W1584465645 abstract "Abstract This unit describes the use of the polymerase chain reaction (PCR) to characterize rearranged murine T cell receptor (TCR) transcripts present in primary lymphoid tissues, peripheral lymphoid tissues, and extra‐lymphoid sites of inflammation, and to quantify them either relatively or absolutely. This methodology has been used extensively to characterize TCR variability in murine thymus and liver in T cell clones, and in extralymphoid tissues where the absolute number of T cells is limited. A procedure for cloning and sequencing PCR‐amplified cDNA transcripts is provided to examine the junctional diversity of expressed genes. Also included is a method for reverse transcription of RNA into cDNA that is optimal for analysis of tissue samples where the number of T cells is limited. The detailed protocols are followed by a commentary that discusses strategies and artifacts as well as providing troubleshooting suggestions for PCR amplification and TCR analysis." @default.
- W1584465645 created "2016-06-24" @default.
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- W1584465645 date "1997-06-01" @default.
- W1584465645 modified "2023-10-10" @default.
- W1584465645 title "Molecular Analysis of Mouse <scp>T</scp> Cell Receptor Expression Using <scp>PCR</scp>" @default.
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- W1584465645 doi "https://doi.org/10.1002/0471142735.im1027s22" @default.
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