Matches in SemOpenAlex for { <https://semopenalex.org/work/W1584494945> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1584494945 endingPage "563" @default.
- W1584494945 startingPage "556" @default.
- W1584494945 abstract "A chromosomal tetracycline resistance (Tcr) determinant previously cloned from Streptococcus mutans into Streptococcus sanguis (Tobian and Macrina, J. Bacteriol. 152:215-222, 1982) was characterized by using restriction endonuclease mapping, deletion analysis, and Southern blot hybridization. Deletion analysis allowed localization of the Tcr determinant to a 2.8-kilobase region of the originally cloned 10.4-kilobase sequence. This cloned determinant hybridized to a representative of the tetM class of streptococcal Tcr determinants but not to representatives of the tetL and tetN classes and, like other tetM determinants, mediated high-level resistance to tetracycline and low-level resistance to minocycline. A portion (approximately 3 kilobases) of the isolated streptococcal fragment was subcloned into Escherichia coli, where it conferred resistance to tetracycline and minocycline. Two proteins with apparent molecular weights of 33,000 and 35,000, encoded by the S. mutans DNA, were synthesized in E. coli minicells. Insertion of DNA into a unique SstI site of the cloned S. mutans fragment resulted in inactivation of Tcr expression in E. coli and S. sanguis, as well as loss of production of both the 33,000- and 35,000-dalton proteins in E. coli minicells. Incubation of minicells in subinhibitory concentrations of tetracycline did not result in changes in the levels of synthesis of either protein. Our data suggest that at least one of these proteins is involved in the expression of Tcr." @default.
- W1584494945 created "2016-06-24" @default.
- W1584494945 creator A5053591574 @default.
- W1584494945 creator A5054189539 @default.
- W1584494945 creator A5077166984 @default.
- W1584494945 date "1984-11-01" @default.
- W1584494945 modified "2023-10-16" @default.
- W1584494945 title "Characterization and expression of a cloned tetracycline resistance determinant from the chromosome of Streptococcus mutans" @default.
- W1584494945 cites W1483121898 @default.
- W1584494945 cites W1484403996 @default.
- W1584494945 cites W1498382819 @default.
- W1584494945 cites W1563315843 @default.
- W1584494945 cites W1606423922 @default.
- W1584494945 cites W1697523668 @default.
- W1584494945 cites W1886895495 @default.
- W1584494945 cites W1898485134 @default.
- W1584494945 cites W1937357034 @default.
- W1584494945 cites W1974474555 @default.
- W1584494945 cites W1974596559 @default.
- W1584494945 cites W1988974352 @default.
- W1584494945 cites W1992042701 @default.
- W1584494945 cites W1995814439 @default.
- W1584494945 cites W1998231415 @default.
- W1584494945 cites W2014610045 @default.
- W1584494945 cites W2015098348 @default.
- W1584494945 cites W2023510572 @default.
- W1584494945 cites W2036528751 @default.
- W1584494945 cites W2051634602 @default.
- W1584494945 cites W2061281406 @default.
- W1584494945 cites W2063450941 @default.
- W1584494945 cites W2069335714 @default.
- W1584494945 cites W2077703362 @default.
- W1584494945 cites W2083503737 @default.
- W1584494945 cites W2121589279 @default.
- W1584494945 cites W2124953058 @default.
- W1584494945 cites W2139174629 @default.
- W1584494945 cites W2159064668 @default.
- W1584494945 cites W2207301048 @default.
- W1584494945 cites W2296946588 @default.
- W1584494945 doi "https://doi.org/10.1128/jb.160.2.556-563.1984" @default.
- W1584494945 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/214770" @default.
- W1584494945 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6094475" @default.
- W1584494945 hasPublicationYear "1984" @default.
- W1584494945 type Work @default.
- W1584494945 sameAs 1584494945 @default.
- W1584494945 citedByCount "34" @default.
- W1584494945 crossrefType "journal-article" @default.
- W1584494945 hasAuthorship W1584494945A5053591574 @default.
- W1584494945 hasAuthorship W1584494945A5054189539 @default.
- W1584494945 hasAuthorship W1584494945A5077166984 @default.
- W1584494945 hasBestOaLocation W15844949451 @default.
- W1584494945 hasConcept C104317684 @default.
- W1584494945 hasConcept C128319531 @default.
- W1584494945 hasConcept C133307689 @default.
- W1584494945 hasConcept C153911025 @default.
- W1584494945 hasConcept C2778430296 @default.
- W1584494945 hasConcept C2780923616 @default.
- W1584494945 hasConcept C501593827 @default.
- W1584494945 hasConcept C523546767 @default.
- W1584494945 hasConcept C54355233 @default.
- W1584494945 hasConcept C547475151 @default.
- W1584494945 hasConcept C552990157 @default.
- W1584494945 hasConcept C86803240 @default.
- W1584494945 hasConcept C89423630 @default.
- W1584494945 hasConceptScore W1584494945C104317684 @default.
- W1584494945 hasConceptScore W1584494945C128319531 @default.
- W1584494945 hasConceptScore W1584494945C133307689 @default.
- W1584494945 hasConceptScore W1584494945C153911025 @default.
- W1584494945 hasConceptScore W1584494945C2778430296 @default.
- W1584494945 hasConceptScore W1584494945C2780923616 @default.
- W1584494945 hasConceptScore W1584494945C501593827 @default.
- W1584494945 hasConceptScore W1584494945C523546767 @default.
- W1584494945 hasConceptScore W1584494945C54355233 @default.
- W1584494945 hasConceptScore W1584494945C547475151 @default.
- W1584494945 hasConceptScore W1584494945C552990157 @default.
- W1584494945 hasConceptScore W1584494945C86803240 @default.
- W1584494945 hasConceptScore W1584494945C89423630 @default.
- W1584494945 hasIssue "2" @default.
- W1584494945 hasLocation W15844949451 @default.
- W1584494945 hasLocation W15844949452 @default.
- W1584494945 hasLocation W15844949453 @default.
- W1584494945 hasOpenAccess W1584494945 @default.
- W1584494945 hasPrimaryLocation W15844949451 @default.
- W1584494945 hasRelatedWork W1968562588 @default.
- W1584494945 hasRelatedWork W1990502365 @default.
- W1584494945 hasRelatedWork W2016821945 @default.
- W1584494945 hasRelatedWork W2113629385 @default.
- W1584494945 hasRelatedWork W2130545881 @default.
- W1584494945 hasRelatedWork W2145368198 @default.
- W1584494945 hasRelatedWork W2158738420 @default.
- W1584494945 hasRelatedWork W2321516259 @default.
- W1584494945 hasRelatedWork W2341696882 @default.
- W1584494945 hasRelatedWork W2740995197 @default.
- W1584494945 hasVolume "160" @default.
- W1584494945 isParatext "false" @default.
- W1584494945 isRetracted "false" @default.
- W1584494945 magId "1584494945" @default.
- W1584494945 workType "article" @default.