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• W1584627230 abstract "Abstract Beginning in the 1980s, automation of DNA sequencing has greatly increased throughput, reduced costs, and enabled large projects to be completed more easily. The development of automation technology paralleled the development of other aspects of DNA sequencing: better enzymes and chemistry, separation and imaging technology, sequencing protocols, robotics, and computational advancements (including base‐calling algorithms with quality scores, database developments, and sequence analysis programs). Despite the emergence of high‐throughput sequencing platforms, automated Sanger sequencing technology remains useful for many applications. This unit provides background and a description of the “First‐Generation” automated DNA sequencing technology. It also includes protocols for using the current Applied Biosystems (ABI) automated DNA sequencing machines. Curr. Protoc. Mol. Biol . 96:7.2.1‐7.2.28. © 2011 by John Wiley & Sons, Inc." @default.
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• W1584627230 date "2011-10-01" @default.
• W1584627230 modified "2023-10-12" @default.
• W1584627230 title "“First Generation” Automated DNA Sequencing Technology" @default.
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• W1584627230 doi "https://doi.org/10.1002/0471142727.mb0702s96" @default.
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