FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1584902893> ?p ?o ?g. }

• W1584902893 endingPage "13819" @default.
• W1584902893 startingPage "13813" @default.
• W1584902893 abstract "The surface coat of Trypanosoma brucei is composed of 10(7) molecules of the variant surface glycoprotein (VSG). Each VSG molecule is tethered to the cell membrane by a glycolipid moiety which contains 1,2-dimyristoyl-sn-phosphatidylinositol (Ferguson, M. A. J., Low, M. G., and Cross, G. A. M. (1985) J. Biol. Chem. 260, 14547-14555). Following cell lysis, an endogenous phospholipase C cleaves dimyristoyl glycerol from the glycolipid, releasing soluble VSG. We have purified this enzyme, which we designate VSG lipase, by detergent extraction, (NH4)2SO4 fractionation, hydrophobic chromatography, and cation exchange chromatography. It is purified 2600-fold and is virtually homogeneous. Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the apparent molecular mass is 37 kDa. In solutions containing the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), the Stokes radius (2.6 nm), S20,w (3.7 S), and v (0.77 cm3/g) of VSG lipase suggest a molecular mass for the native enzyme of about 47 kDa, part of which may be due to bound CHAPS. Therefore, it is probably monomeric. VSG lipase does not require Ca2+; it is stimulated by chelating agents or dithiothreitol, and it is inhibited by some sulfhydryl reagents. The purified enzyme appears to be highly specific. Under the conditions of our assay, it cleaves the VSG glycolipid, a biosynthetic precursor of the VSG glycolipid, and, to a much lesser extent, 1,2-dimyristoyl-sn-phosphatidylinositol. There was no apparent cleavage of other myristate-containing lipids of trypanosomes or 1-stearoyl-2-arachidonoyl-sn-phosphatidylinositol." @default.
• W1584902893 created "2016-06-24" @default.
• W1584902893 creator A5029327136 @default.
• W1584902893 creator A5043863262 @default.
• W1584902893 creator A5067016480 @default.
• W1584902893 creator A5072571167 @default.
• W1584902893 creator A5090809624 @default.
• W1584902893 date "1986-10-01" @default.
• W1584902893 modified "2023-09-27" @default.
• W1584902893 title "A phospholipase C from Trypanosoma brucei which selectively cleaves the glycolipid on the variant surface glycoprotein." @default.
• W1584902893 cites W1482401645 @default.
• W1584902893 cites W1486467708 @default.
• W1584902893 cites W1501502413 @default.
• W1584902893 cites W1528089915 @default.
• W1584902893 cites W1551098723 @default.
• W1584902893 cites W1571818177 @default.
• W1584902893 cites W1604033226 @default.
• W1584902893 cites W1654759002 @default.
• W1584902893 cites W1775749144 @default.
• W1584902893 cites W1808269271 @default.
• W1584902893 cites W1875052998 @default.
• W1584902893 cites W1896631934 @default.
• W1584902893 cites W1906639847 @default.
• W1584902893 cites W192249699 @default.
• W1584902893 cites W1964237109 @default.
• W1584902893 cites W1966336919 @default.
• W1584902893 cites W1970522510 @default.
• W1584902893 cites W1979492674 @default.
• W1584902893 cites W1985811428 @default.
• W1584902893 cites W1989054182 @default.
• W1584902893 cites W1992760440 @default.
• W1584902893 cites W1992799821 @default.
• W1584902893 cites W1996827534 @default.
• W1584902893 cites W2016686298 @default.
• W1584902893 cites W2019934106 @default.
• W1584902893 cites W2020097304 @default.
• W1584902893 cites W2025088763 @default.
• W1584902893 cites W2027097213 @default.
• W1584902893 cites W2027930479 @default.
• W1584902893 cites W2029006593 @default.
• W1584902893 cites W2035428685 @default.
• W1584902893 cites W2036911556 @default.
• W1584902893 cites W2050852542 @default.
• W1584902893 cites W2058793271 @default.
• W1584902893 cites W2061021706 @default.
• W1584902893 cites W2086869480 @default.
• W1584902893 cites W2089102363 @default.
• W1584902893 cites W2100837269 @default.
• W1584902893 cites W2109971460 @default.
• W1584902893 cites W2111691978 @default.
• W1584902893 cites W2119460480 @default.
• W1584902893 cites W2155322008 @default.
• W1584902893 cites W2156691397 @default.
• W1584902893 cites W2177660864 @default.
• W1584902893 cites W2303269923 @default.
• W1584902893 cites W4211175188 @default.
• W1584902893 cites W51420773 @default.
• W1584902893 doi "https://doi.org/10.1016/s0021-9258(18)67092-9" @default.
• W1584902893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3759991" @default.
• W1584902893 hasPublicationYear "1986" @default.
• W1584902893 type Work @default.
• W1584902893 sameAs 1584902893 @default.
• W1584902893 citedByCount "190" @default.
• W1584902893 countsByYear W15849028932013 @default.
• W1584902893 countsByYear W15849028932014 @default.
• W1584902893 countsByYear W15849028932015 @default.
• W1584902893 countsByYear W15849028932016 @default.
• W1584902893 countsByYear W15849028932017 @default.
• W1584902893 countsByYear W15849028932018 @default.
• W1584902893 countsByYear W15849028932020 @default.
• W1584902893 countsByYear W15849028932021 @default.
• W1584902893 countsByYear W15849028932022 @default.
• W1584902893 countsByYear W15849028932023 @default.
• W1584902893 crossrefType "journal-article" @default.
• W1584902893 hasAuthorship W1584902893A5029327136 @default.
• W1584902893 hasAuthorship W1584902893A5043863262 @default.
• W1584902893 hasAuthorship W1584902893A5067016480 @default.
• W1584902893 hasAuthorship W1584902893A5072571167 @default.
• W1584902893 hasAuthorship W1584902893A5090809624 @default.
• W1584902893 hasBestOaLocation W15849028931 @default.
• W1584902893 hasConcept C104317684 @default.
• W1584902893 hasConcept C108625454 @default.
• W1584902893 hasConcept C12927208 @default.
• W1584902893 hasConcept C159047783 @default.
• W1584902893 hasConcept C181199279 @default.
• W1584902893 hasConcept C185592680 @default.
• W1584902893 hasConcept C22222904 @default.
• W1584902893 hasConcept C2778597717 @default.
• W1584902893 hasConcept C2779502636 @default.
• W1584902893 hasConcept C2779549974 @default.
• W1584902893 hasConcept C55493867 @default.
• W1584902893 hasConcept C86803240 @default.
• W1584902893 hasConceptScore W1584902893C104317684 @default.
• W1584902893 hasConceptScore W1584902893C108625454 @default.
• W1584902893 hasConceptScore W1584902893C12927208 @default.
• W1584902893 hasConceptScore W1584902893C159047783 @default.
• W1584902893 hasConceptScore W1584902893C181199279 @default.
• W1584902893 hasConceptScore W1584902893C185592680 @default.

• next