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- W1584964960 abstract "Abstract The Ku antigen is a DNA-associated nuclear protein recognized by sera from patients with autoimmune diseases. It consists of two polypeptides of 86 and 70 kDa. cDNA clones encoding the 86-kDa subunit of the Ku antigen were isolated by probing lambda gt11 recombinant cDNA expression libraries with a monoclonal antibody specific for this protein. The amino acid sequence deduced from the cDNA comprises 732 amino acids and corresponds to a protein with molecular weight of 81.914. Nineteen residues at the NH2 terminus determined by protein sequencing corresponded to the sequence deduced from the cDNA. The predicted amino acid sequence contains a region with repeating residues similar to the leucine zipper structure observed in the c-myc, v-myc, and c-fos oncogene products. The largest cDNA hybridized to 2.7- and 3.4-kilobase poly(A)+ mRNAs from HeLa cells. The cDNA clones expressed fusion proteins immunoreactive with the monoclonal antibody and sera from patients with autoimmune diseases." @default.
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- W1584964960 date "1989-08-01" @default.
- W1584964960 modified "2023-10-18" @default.
- W1584964960 title "cDNA-derived Amino Acid Sequence of the 86-kDa Subunit of the Ku Antigen" @default.
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- W1584964960 doi "https://doi.org/10.1016/s0021-9258(18)80011-4" @default.
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