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- W1585449363 abstract "The size of the Ca2+ pump protein in canine cardiac sarcoplasmic reticulum (SR) membranes was determined using target theory analysis of radiation-inactivation data. Samples of cardiac SR were irradiated in the frozen state with increasing doses of high energy electrons from a Van de Graaff accelerator. The loss of Ca2+-dependent ATPase activity and of ATP-dependent, oxalate-facilitated Ca2+ uptake (loading) with increasing irradiation dosage paralleled one another. Also, the loss of staining intensity of the predominant polypeptide (Mr = 110,000) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis correlated with the loss of ATPase and loading activities. The target size by all three measurements varied between 213,000 and 229,000 Da. We conclude that the Ca2+ pump protein in canine cardiac SR is a dimer." @default.
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- W1585449363 date "1983-10-01" @default.
- W1585449363 modified "2023-09-28" @default.
- W1585449363 title "Determination of the oligomeric structure of the Ca2+ pump protein in canine cardiac sarcoplasmic reticulum membranes using radiation inactivation analysis." @default.
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- W1585449363 doi "https://doi.org/10.1016/s0021-9258(17)44331-6" @default.
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