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- W1585753614 abstract "13,14-Dihydro-15-ketoprostaglandin (PG) F2 alpha was separated completely from PGD2, PGE2 and PGF2 alpha within 10-15 min by gas chromatography-selected-ion monitoring on a capillary column after conversion to their methyl ester-n-butyloxime-dimethylisopropylsilyl (ME-nBO-DMiPS) ether or ME-nBO-cyclic diethylsilylene (DES) derivatives. The mass spectra were characterized by their inherent ions of [M-43]+ at m/z 596 for the ME-nBO-DMiPS ether and of [M-73]+ at m/z 450 for the ME-nBO-DES derivatives, respectively. The appearance of the characteristic ions in the high-mass region with relatively high intensity and the use of these ions in selected-ion monitoring in a high-resolution mode (M/delta M = 3500) made it possible to enhance the selectivity in the detection of 13,14-dihydro-15-keto-PGF2 alpha in biological fluids. The detection limit of the ME-nBO-DES derivative of 13,14-dihydro-15-keto-PGF2 alpha was found to be 2 pg with a signal-to-noise ratio of 4:1 when the ion of [M-73]+ was monitored at m/z 450.30." @default.
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- W1585753614 date "1987-07-24" @default.
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- W1585753614 title "Gas chromatography-mass spectrometry of some prostanoids with new derivatizing agents. Application to the analysis of 13,14-dihydro-15-ketoprostaglandin F2 alpha." @default.
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