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- W1586076218 abstract "Several aspects of the preparation of homogenates and certain physical properties of suspending media have been examined in an attempt to develop a more rational approach to the problem of the differential centrifugation of tissue homogenates. The addition of calcium-binding agents to the perfusion solution has been found to result in the presence of a large number of whole cells in liver breis. No difference in cell breakage was observed when calcium-free Locke's solution was compared with complete Locke's solution. The addition of 0.0018 M CaCl2 to a perfusion medium consisting of 0.25 M sucrose resulted in an increase in the number of whole cells and cell wall material when compared with sucrose alone. Comparison of the composition of homogenates identically prepared except for variations in the pH of the homogenization medium showed that at pH 6 a large number of whole cells, broken cells, and cell wall fragments were present. The number of these contaminants decreased markedly as the pH was raised to 7.88. Data on the viscosity and density of a number of suspending media, on the density of perfused liver, and on the density of homogenates are presented. From these the requirements for the preparation of layered systems for differential centrifugation can be calculated." @default.
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- W1586076218 date "1955-01-01" @default.
- W1586076218 modified "2023-10-18" @default.
- W1586076218 title "Studies on isolated cell components" @default.
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- W1586076218 doi "https://doi.org/10.1016/0014-4827(55)90045-8" @default.
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