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- W1586199201 abstract "The relative fluorescence yield of catecholamines after condensation with formaldehyde according to the histochemical technique of Falck and Hillarp has been investigated in solution and in model systems, the latter in order to imitate the situation in freeze-dried tissues. The models consisted of catecholamines dissolved in a sucrose or protein solution, which was sprayed onto object slides to form dried micro droplets. After formaldehyde treatment according to the histochemical technique, the models were examined with respect to absorption and emission characteristics, reaction conditions, and influence of water in the specimens. Special attention was paid to the relation of the fluorescence intensity to the concentration of the fluorophore. It was found that the linear relationship between the two at low concentrations was broken at around 4.5 × 10−2 moles/1 dried droplet volume. Above this concentration, a further increase in catecholamine concentration was not accompanied by a corresponding increase in fluorescence intensity, possibly due to intermolecular energy transfers between the relatively closely packed molecules. This concentration quenching should be kept in mind when trying to interpret fluorescence intensity as a measure of catecholamine concentration. It is pointed out that a similar concentration quenching is likely to appear also in other cytochemical fluorescence techniques where the concentrations often are much higher than in the commonly used biochemical fluorescence assays. This error is eliminated if the distance between the fluorescent molecules is kept constant." @default.
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- W1586199201 date "1966-11-01" @default.
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- W1586199201 title "Quantitative fluorescence microspectrophotometry of catecholamine-formaldehyde products" @default.
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- W1586199201 doi "https://doi.org/10.1016/0014-4827(66)90456-3" @default.
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