FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1586201322> ?p ?o ?g. }

• W1586201322 endingPage "2165" @default.
• W1586201322 startingPage "2160" @default.
• W1586201322 abstract "Abstract Topoisomerase II (Top II) is the target enzyme for many antineoplastic drugs such as epipodophyllotoxins, anthracyclines, and acridines. Cell lines with alterations in Top II are resistant to drugs that interact with the enzyme. Studies of the Top II from a Chinese hamster ovary line, VpmR-5, that is resistant to VP-16 and VM-26, demonstrated that it is very similar, qualitatively and quantitatively, to its normal counterpart except that DNA cleavage by the VpmR-5 enzyme is not stimulated by VP-16 or VM-26. To understand the basis for the drug-resistant phenotype, the Top II cDNAs were isolated from both Chinese hamster ovary (CHO) and VpmR-5 cells by cDNA cloning with lambda gt22, and the entire cDNAs were sequenced. A mutation of G-->A at nucleotide 1478 was the only alteration observed in the VpmR-5 Top II cDNA compared with the wild-type gene. The mutation in VpmR-5 was confirmed by sequencing DNA fragments amplified from the genomic DNA by the polymerase chain reaction. Southern blot hybridization analysis of genomic DNA demonstrated loss of a Top II allele in VpmR-5 probably occurred during the development of resistance to etoposide. The mutation in VpmR-5 changes amino acid 493 from arginine to glutamine and is located adjacent to a putative ATP binding site of Top II. Mutations in an analogous region have been identified in two human leukemia cell lines by amplification of segments of Top II cDNA with Taq DNA polymerase. Taken together, these observations suggest that mutations in this region of the gyrase B domain of mammalian topoisomerase II may be capable of conferring resistance to antineoplastic agents that interact with this enzyme." @default.
• W1586201322 created "2016-06-24" @default.
• W1586201322 creator A5003908210 @default.
• W1586201322 creator A5040804506 @default.
• W1586201322 creator A5045982804 @default.
• W1586201322 creator A5080591345 @default.
• W1586201322 date "1993-01-01" @default.
• W1586201322 modified "2023-10-16" @default.
• W1586201322 title "Molecular cloning and identification of a point mutation in the topoisomerase II cDNA from an etoposide-resistant Chinese hamster ovary cell line." @default.
• W1586201322 cites W1491255035 @default.
• W1586201322 cites W1555798239 @default.
• W1586201322 cites W1559338540 @default.
• W1586201322 cites W1602013545 @default.
• W1586201322 cites W1973416721 @default.
• W1586201322 cites W1976057490 @default.
• W1586201322 cites W1977754686 @default.
• W1586201322 cites W1982534799 @default.
• W1586201322 cites W1986490393 @default.
• W1586201322 cites W1989377878 @default.
• W1586201322 cites W1993553978 @default.
• W1586201322 cites W1997789257 @default.
• W1586201322 cites W2002157448 @default.
• W1586201322 cites W2011106770 @default.
• W1586201322 cites W2012754297 @default.
• W1586201322 cites W2020257412 @default.
• W1586201322 cites W2021740675 @default.
• W1586201322 cites W2026002774 @default.
• W1586201322 cites W2042367294 @default.
• W1586201322 cites W2044633392 @default.
• W1586201322 cites W2047632936 @default.
• W1586201322 cites W2050717506 @default.
• W1586201322 cites W2058911936 @default.
• W1586201322 cites W2060268336 @default.
• W1586201322 cites W2060333964 @default.
• W1586201322 cites W2066160869 @default.
• W1586201322 cites W2078035031 @default.
• W1586201322 cites W2083153561 @default.
• W1586201322 cites W2123126235 @default.
• W1586201322 cites W4233567369 @default.
• W1586201322 doi "https://doi.org/10.1016/s0021-9258(18)53976-4" @default.
• W1586201322 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8380592" @default.
• W1586201322 hasPublicationYear "1993" @default.
• W1586201322 type Work @default.
• W1586201322 sameAs 1586201322 @default.
• W1586201322 citedByCount "59" @default.
• W1586201322 countsByYear W15862013222013 @default.
• W1586201322 crossrefType "journal-article" @default.
• W1586201322 hasAuthorship W1586201322A5003908210 @default.
• W1586201322 hasAuthorship W1586201322A5040804506 @default.
• W1586201322 hasAuthorship W1586201322A5045982804 @default.
• W1586201322 hasAuthorship W1586201322A5080591345 @default.
• W1586201322 hasBestOaLocation W15862013221 @default.
• W1586201322 hasConcept C104317684 @default.
• W1586201322 hasConcept C121050878 @default.
• W1586201322 hasConcept C147897179 @default.
• W1586201322 hasConcept C153911025 @default.
• W1586201322 hasConcept C175656101 @default.
• W1586201322 hasConcept C176944494 @default.
• W1586201322 hasConcept C187882448 @default.
• W1586201322 hasConcept C199360897 @default.
• W1586201322 hasConcept C2776694085 @default.
• W1586201322 hasConcept C2778119113 @default.
• W1586201322 hasConcept C41008148 @default.
• W1586201322 hasConcept C501734568 @default.
• W1586201322 hasConcept C54355233 @default.
• W1586201322 hasConcept C552990157 @default.
• W1586201322 hasConcept C81885089 @default.
• W1586201322 hasConcept C86803240 @default.
• W1586201322 hasConceptScore W1586201322C104317684 @default.
• W1586201322 hasConceptScore W1586201322C121050878 @default.
• W1586201322 hasConceptScore W1586201322C147897179 @default.
• W1586201322 hasConceptScore W1586201322C153911025 @default.
• W1586201322 hasConceptScore W1586201322C175656101 @default.
• W1586201322 hasConceptScore W1586201322C176944494 @default.
• W1586201322 hasConceptScore W1586201322C187882448 @default.
• W1586201322 hasConceptScore W1586201322C199360897 @default.
• W1586201322 hasConceptScore W1586201322C2776694085 @default.
• W1586201322 hasConceptScore W1586201322C2778119113 @default.
• W1586201322 hasConceptScore W1586201322C41008148 @default.
• W1586201322 hasConceptScore W1586201322C501734568 @default.
• W1586201322 hasConceptScore W1586201322C54355233 @default.
• W1586201322 hasConceptScore W1586201322C552990157 @default.
• W1586201322 hasConceptScore W1586201322C81885089 @default.
• W1586201322 hasConceptScore W1586201322C86803240 @default.
• W1586201322 hasIssue "3" @default.
• W1586201322 hasLocation W15862013221 @default.
• W1586201322 hasOpenAccess W1586201322 @default.
• W1586201322 hasPrimaryLocation W15862013221 @default.
• W1586201322 hasRelatedWork W1523914420 @default.
• W1586201322 hasRelatedWork W1586201322 @default.
• W1586201322 hasRelatedWork W1980092410 @default.
• W1586201322 hasRelatedWork W2050101539 @default.
• W1586201322 hasRelatedWork W2083390593 @default.
• W1586201322 hasRelatedWork W2180821465 @default.
• W1586201322 hasRelatedWork W2410470679 @default.
• W1586201322 hasRelatedWork W2416035794 @default.
• W1586201322 hasRelatedWork W2416358175 @default.
• W1586201322 hasRelatedWork W2606394413 @default.

• next