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• W1587065587 abstract "Background Maternal immunization against KEL 1 of the K ell blood group system can have serious adverse consequences for the fetus as well as the newborn baby. Therefore, it is important to determine the phenotype of the fetus to predict whether it is at risk. We present data that show the feasibility of predicting the fetal KEL 1 phenotype using next‐generation sequencing ( NGS ) technology. Study Design and Methods The KEL 1/2 single‐nucleotide polymorphism was polymerase chain reaction ( PCR ) amplified with one adjoining base, and the PCR product was sequenced using a genome analyzer ( GAIIx , Illumina); several millions of PCR sequences were analyzed. Results The results demonstrated the feasibility of diagnosing the fetal KEL 1 or KEL 2 blood group from cell‐free DNA purified from maternal plasma. Conclusion This method requires only one primer pair, and the large amount of sequence information obtained allows well for statistical analysis of the data. This general approach can be integrated into current laboratory practice and has numerous applications. Besides DNA ‐based predictions of blood group phenotypes, platelet phenotypes, or sickle cell anemia, and the determination of zygosity, various conditions of chimerism could also be examined using this approach. To our knowledge, this is the first report focused on antenatal blood group determination using NGS ." @default.
• W1587065587 created "2016-06-24" @default.
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• W1587065587 date "2013-04-03" @default.
• W1587065587 modified "2023-10-09" @default.
• W1587065587 title "Next-generation sequencing: proof of concept for antenatal prediction of the fetal Kell blood group phenotype from cell-free fetal DNA in maternal plasma" @default.
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• W1587065587 doi "https://doi.org/10.1111/trf.12172" @default.
• W1587065587 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23550721" @default.
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